BD Bbl™ Oxidase Reagent Droppers

The BD BBL™ Oxidase Reagent Droppers (261181) provide a reliable and convenient solution for the oxidase test, a critical procedure for identifying and differentiating oxidase-positive bacteria such as Pseudomonas, Neisseria, and Campylobacter. These reagent droppers contain 1% tetramethyl-p-phenylenediamine dihydrochloride, a reagent that facilitates the detection of the cytochrome c oxidase enzyme in bacterial colonies.

Key Features

1. Ready-to-Use Format:
   • Pre-filled droppers simplify reagent handling and minimize preparation time.
2. Convenience:
   • Each dropper is designed for single-use, ensuring accuracy and avoiding cross-contamination.
3. Stable and Reliable:
   • The reagent is stable and provides consistent results for oxidase testing.
4. Easy Application:
   • The droppers allow for direct application of the reagent onto bacterial colonies, reducing procedural complexity.
5. Colorimetric Detection:
   • Positive reactions produce a distinct purple color, indicating the presence of cytochrome c oxidase.

Specifications

Applications

1. Microbial Identification:

• Used to identify oxidase-positive bacteria in clinical, environmental, and food microbiology laboratories.

2. Differentiation of Bacteria:

• Distinguishes oxidase-positive bacteria (Pseudomonas, Neisseria, Campylobacter) from oxidase-negative bacteria (Enterobacteriaceae).

3. Clinical Diagnostics:

• Facilitates the identification of pathogens in clinical specimens, aiding in the diagnosis of bacterial infections.

4. Educational Use:

• Ideal for teaching microbiology and bacteriology techniques in academic settings.

Testing Procedure

1. Sample Preparation:
   • Grow bacterial colonies on a suitable agar medium, such as blood agar or nutrient agar.
   • Ensure the colonies are fresh, as older colonies may yield unreliable results.
2. Application:
   • Remove a single-use dropper and apply the reagent directly onto the bacterial colony or a filter paper with bacterial growth.
3. Observation:
   • Observe for a color change within 10–30 seconds.
   • Positive Result: Purple color indicates the presence of cytochrome c oxidase.
   • Negative Result: No color change.
4. Disposal:
   • Discard used droppers safely according to laboratory protocols.

Advantages of BD BBL™ Oxidase Reagent Droppers

1. Convenience:
   • Single-use droppers eliminate the need for reagent preparation, reducing handling time and contamination risks.
2. Accuracy:
   • Provides reliable and reproducible results for oxidase testing.
3. Ease of Use:
   • Simplifies workflow with a ready-to-use format suitable for various laboratory settings.
4. Enhanced Safety:
   • Pre-packaged, sterile droppers ensure reagent integrity and minimize exposure to hazardous chemicals.

Storage and Handling

• Storage Conditions: Store at 2–8°C to maintain reagent stability.
• Shelf Life: Use before the expiration date indicated on the packaging.
• Safety Precautions:
  • Handle reagents with care, and follow laboratory safety protocols.
  • Dispose of used droppers according to biohazard waste guidelines.

Why Choose BD BBL™ Oxidase Reagent Droppers?

• Trusted Brand: Backed by BD, a global leader in microbiology and laboratory diagnostics.
• Time-Saving: Ready-to-use droppers streamline workflows and reduce preparation steps.
• Accurate Results: Ensures precise and reliable oxidase testing for accurate bacterial identification.

Conclusion

The BD BBL™ Oxidase Reagent Droppers (261181) are an essential tool for microbiologists and clinicians seeking a convenient, reliable, and accurate solution for oxidase testing. With their ready-to-use format, stable formulation, and trusted BD quality, these droppers simplify the identification and differentiation of oxidase-positive bacteria in diverse research and clinical applications.