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Thermo Scientific™ Pierce™ NHS (N-hydroxysuccinimide), 25g
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₄H₅NO₃ |
Thermo Scientific™ Pierce™ NHS (N-hydroxysuccinimide) is a high-purity chemical modification reagent used to activate carboxyl groups (—COOH) for conjugation with primary amines (—NH₂), enabling efficient bioconjugation, crosslinking, labeling, and immobilization of biomolecules. NHS is widely used in protein chemistry, antibody conjugation, peptide labeling, and surface functionalization workflows, particularly in combination with carbodiimide crosslinkers such as EDC.
NHS reacts with carboxyl-containing molecules to form stable, amine-reactive NHS esters, facilitating highly efficient covalent coupling reactions. This process improves crosslinking efficiency, increases reaction control, and produces stable conjugates for applications in molecular biology, immunoassays, diagnostics research, and biomaterials development.
As the uncharged analog of Sulfo-NHS, Thermo Scientific Pierce NHS offers enhanced solubility in organic solvents such as DMSO, allowing better membrane permeability and compatibility with hydrophobic molecules. Its high purity crystalline form ensures reliable activation and consistent conjugation efficiency in demanding laboratory applications.
Key Features and Benefits
- Converts carboxyl groups into highly reactive NHS esters
- Enables efficient amine-reactive bioconjugation and crosslinking
- Improves efficiency of EDC-mediated coupling reactions
- Suitable for proteins, peptides, antibodies, and biomolecules
- High-purity crystalline reagent for consistent results
- Compatible with organic solvents such as DMSO
- Supports labeling, immobilization, and surface modification applications
- Reliable reagent for molecular biology and biochemical research
Applications
- Protein and antibody conjugation
- Bioconjugation and crosslinking reactions
- Peptide labeling and modification
- Immobilization of biomolecules on surfaces
- Immunoassay development (ELISA, lateral flow assays)
- Biosensor functionalization
- Surface chemistry and biomaterials research
- Chemical labeling and molecular modification
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | N-hydroxysuccinimide (NHS) |
| Catalog Number | 24500 |
| Quantity | 25 g |
| Product Line | Pierce™ |
| Form | Crystalline powder |
| Molecular Weight | 115.09 g/mol |
| Reactive Groups | Carboxyl-reactive, amine coupling |
| Crosslinker Type | Carbodiimide crosslinking reagent |
| Spacer Length | Short (<10 Å) |
| Solubility | Water soluble; also soluble in DMSO |
| Labeling Method | Chemical labeling |
| PEGylated | No |
| Cleavable | No |
| Shipping Condition | Ambient |
| Storage Condition | Store at 4°C |
| Unit Size | Each |
Ordering Information
| Catalog Number | Product Name | Quantity | Brand |
|---|---|---|---|
| 24500 | Thermo Scientific™ Pierce™ NHS (N-hydroxysuccinimide) | 25 g | Thermo Scientific |
Thermo Scientific Pierce NHS is an essential reagent for researchers performing bioconjugation and crosslinking reactions. Its ability to efficiently activate carboxyl groups for amine coupling makes it ideal for protein conjugation, antibody labeling, and biomolecule immobilization. With high purity, reliable performance, and compatibility with carbodiimide chemistry, NHS is a trusted reagent in molecular biology, biotechnology, and biochemical research laboratories.