Gibco™ PFHM-II Protein-Free Hybridoma Medium (powder), 10L

Gibco™ PFHM-II Protein-Free Hybridoma Medium (Powder) is a serum-free, protein-free, and animal origin–free medium specifically formulated to support the growth of hybridomas and myelomas for monoclonal antibody production. Offered in an easy-to-reconstitute powder format, this medium is ideal for laboratories seeking scalable, reproducible culture systems with simplified downstream purification.

Upon reconstitution, PFHM-II becomes a complete, ready-to-use medium requiring no additional supplementation. It contains L-glutamine and phenol red and is suitable for both suspension and stationary cultures, including cholesterol-dependent lines like NS0 (with the addition of 250× Cholesterol Lipid Concentrate).

Manufactured in a cGMP-compliant, FDA-registered, and ISO 13485–certified facility, PFHM-II powder ensures consistency, quality, and traceability, making it a suitable choice for regulated research and preclinical manufacturing.

Key Features

• Powder Format for 10 L: Cost-effective and scalable format for high-volume culture.
• Protein-Free and Serum-Free: Enables simplified purification of monoclonal antibodies.
• Animal Origin-Free (AOF): Reduces risk of variability and contamination.
• Complete Upon Reconstitution: Includes L-glutamine and phenol red; no further supplementation required.
• Supports High Cell Densities: Promotes robust growth and IgG production from hybridoma and myeloma lines.

Applications

• Monoclonal Antibody Production:
  • Scalable system for high-yield antibody production using hybridomas or recombinant myelomas.
• Cholesterol-Dependent Cell Lines:
  • Supports NS0 and similar lines with cholesterol supplementation.
• Regulated Bioprocessing:
  • Suitable for FDA submissions; Type II DMF support available.
• Streamlined Purification:
  • Protein-free formulation facilitates downstream protein recovery and analysis.

Specifications

Gibco™ PFHM-II Protein-Free Hybridoma Medium (Powder) provides a reliable, scalable solution for serum-free, high-performance hybridoma and myeloma culture. With a protein-free formulation, pre-included glutamine, and ready-to-use format post-reconstitution, it's ideal for researchers focused on mAb production and purification efficiency under consistent and regulated conditions.