Thermo Scientific™ Immobilized Pepsin (Agarose Resin), 5mL

Thermo Scientific™ Pierce™ Immobilized Pepsin Agarose is a highly effective enzymatic reagent consisting of acidic endopeptidase (pepsin) covalently coupled to crosslinked agarose beads. Designed primarily for controlled proteolysis of immunoglobulins, this immobilized format offers efficient generation of F(ab')₂ antibody fragments while minimizing autolysis and enzyme contamination.

The immobilization of pepsin enables precise control over digestion reactions, simplifies enzyme removal post-digestion, and improves thermal stability. This makes it an excellent tool for antibody fragment preparation, proteomic workflows, and other enzymatic cleavage applications under acidic conditions.

Key Features & Benefits

• Controlled Antibody Digestion
  – Efficiently produces F(ab')₂ fragments by cleaving below the hinge region of IgG antibodies.
• No Enzyme Contamination
  – Immobilization prevents leaching of pepsin into your sample, preserving product purity.
• Resin-Based Format
  – Allows easy separation of enzyme from reaction mixture by simple centrifugation or filtration.
• Enhanced Stability & Activity
  – More resistant to heat-induced denaturation and proteolytic degradation than soluble pepsin.
• Reduced Autolysis
  – Immobilization eliminates self-digestion of the enzyme, extending usability and reducing background noise.

Enzyme Characteristics

Applications

• Antibody Fragmentation
  – Generate F(ab')₂ fragments from IgG for ELISA, Western blot, or immunoassays.
• Proteomic Workflows
  – Controlled cleavage of proteins for mapping studies or structural analysis.
• Immunohistochemistry
  – Prepare antibody fragments to improve tissue penetration and reduce background signal.
• Therapeutic Antibody Development
  – Produce consistent F(ab')₂ fragments for research or preclinical studies.

Recommended Usage

• Use under acidic conditions (pH 1–3) for optimal enzymatic activity.
• Combine with appropriate buffers and incubation protocols based on species (human, rabbit, mouse).
• Remove the immobilized pepsin by simple centrifugation or column filtration to stop the reaction.

Related Products

• Pierce™ F(ab')₂ Preparation Kit – Includes immobilized pepsin, buffers, and protocols for standardized antibody fragmentation.
• Pierce™ Immobilized Papain Resin – For generating Fab fragments by cleavage above the hinge region.

Pierce™ Immobilized Pepsin Agarose is a reliable, convenient, and highly efficient solution for antibody digestion and controlled proteolysis in research applications. Its immobilized format ensures easy handling, clean sample preparation, and minimal contamination—making it ideal for downstream workflows in immunology, proteomics, and antibody engineering.