Thermo Scientific™ Trypsin, TPCK Treated, 50mg

The Thermo Scientific Pierce TPCK Trypsin is a highly specific serine endoprotease treated with TPCK (L-1-tosylamido-2-phenylethyl chloromethyl ketone) to inhibit contaminating chymotrypsin activity while preserving trypsin's enzymatic function. It is widely used for applications in protein sequencing, structural studies, and amino acid analysis, offering precision in peptide bond cleavage.

Key Features

1. Selective Cleavage:
   • Cleaves peptide bonds on the carboxyl side of arginine and lysine residues.
   • Ensures precise digestion for protein analysis.
2. Optimal Digestion Conditions:
   • pH range: 7.5–9.0.
   • Temperature: 37°C.
3. Advantages of TPCK Treatment:
   • Prevents chymotrypsin contamination.
   • Reduces autolysis for consistent activity.
4. Immobilized Trypsin Option:
   • Facilitates sample separation post-digestion.
   • Reduces contamination and enhances thermal stability.

Applications

1. Protein Sequencing and Analysis:
   • Prepare tryptic fragments for Edman degradation sequencing.
   • Protein mapping and structural studies.
2. Cell Culture:
   • Removal of adherent cells from tissue culture flasks.
3. Enzyme Purification:
   • Purification of soybean trypsin inhibitor using immobilized trypsin.
4. Mass Spectrometry:
   • Use for mass spectrometry workflows (recommended: Pierce Trypsin, MS Grade).

Preparation and Storage

1. Preparation:
   • Dissolve 0.25 g of trypsin in 100 mL of chilled HBSS to make a 0.25% w/v solution.
   • Mix until homogeneous and ensure pH is within 7.4–7.8.
   • Filter through a low protein-binding filter (e.g., Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with PES Membranes).
2. Storage:
   • Store at 2–8°C in the original container, away from direct sunlight and in a dry, cool, ventilated area.

Specifications

Related Products

1. Immobilized Trypsin, TPCK Treated:
   • Agarose resin-based trypsin for reduced contamination and easier separation.
2. Pierce Trypsin, MS Grade (Cat No. 90057):
   • Optimized for mass spectrometry workflows.

Key Advantages

• Specificity: Ensures accurate cleavage of arginine and lysine residues.
• Stability: TPCK treatment reduces chymotrypsin contamination and autolysis.
• Versatility: Suitable for a wide range of biochemical and molecular biology applications.
• Convenience: Available in powder form for easy preparation.

The Thermo Scientific Pierce TPCK Trypsin is an essential tool for researchers requiring precise enzymatic cleavage for downstream protein and peptide analyses.

Comparison Between Thermo Scientific™ Immobilized Trypsin, TPCK Treated and Trypsin, TPCK Treated

Key Advantages

Immobilized Trypsin, TPCK Treated

• Ease of Separation: Enzyme removal is straightforward post-digestion using centrifugation or filtration.
• Stability: Immobilization enhances stability and reduces heat-induced denaturation.
• Minimal Contamination: Keeps samples protease-free after digestion.
• Reusability: Resin can be reused, making it cost-effective for certain applications.

Trypsin, TPCK Treated

• Flexibility: Powder form allows solution-based reactions with no format restrictions.
• Versatility: Suitable for quick, single-use experiments where enzyme separation is not required.
• Simpler Preparation: Directly dissolvable in buffer for immediate use.

When to Choose Each

Conclusion

• Immobilized Trypsin, TPCK Treated is ideal for workflows requiring sample purity, reduced contamination, and reusability, especially in structural studies or affinity purification.
• Trypsin, TPCK Treated is preferred for versatile, solution-based applications where enzyme removal is not critical.

Your choice depends on whether you prioritize ease of separation and stability (Immobilized Trypsin) or flexibility and versatility (Trypsin in powder form).