Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin), 2mL

The Thermo Scientific Pierce Immobilized TPCK Trypsin is a serine endoprotease immobilized on agarose resin. Designed for amino acid analysis, protein sequencing, and structural studies, this immobilized form offers key advantages such as minimized autolysis, reduced sample contamination, and enhanced stability. The immobilized format allows for easy sample separation after digestion or affinity purification of trypsin inhibitors.

Key Features

1. Selective Cleavage:
   • Cleaves peptide bonds at the carboxyl side of arginine and lysine residues.
   • Offers high specificity, regardless of peptide length or sequence.
2. Immobilized Format Advantages:
   • Allows easy separation of trypsin from cleavage products.
   • Reduces autolysis, extending the enzyme's usability.
   • Prevents contamination of samples with the enzyme.
3. Enhanced Stability:
   • More resistant to heat-induced denaturation.
   • Maintains enzymatic activity for longer durations compared to free trypsin.
4. Optimal Digestion Conditions:
   • pH: 7.5–9.0.
   • Temperature: 37°C.

Applications

1. Protein Sequencing and Analysis:
   • Preparation of tryptic fragments for Edman degradation sequencing.
   • Structural studies and peptide mapping.
2. Cell Culture:
   • Removal of adherent cells from tissue culture flasks.
3. Affinity Purification:
   • Purify soybean trypsin inhibitors using immobilized trypsin.
4. Mass Spectrometry Workflows:
   • Recommended for downstream applications with Pierce Trypsin, MS Grade (Part No. 90057).

Technical Specifications

Preparation and Usage

1. Preparation:
   • Supplied as a 50% slurry.
   • Ready-to-use for digestion workflows; adjust buffer and pH as required.
2. Digestion Protocol:
   • Incubate samples with immobilized trypsin at pH 7.5–9.0 and 37°C.
   • After digestion, separate the enzyme from cleavage products using standard centrifugation or filtration methods.

Key Advantages

1. Minimized Autolysis:
   • Immobilization prevents self-digestion, preserving enzyme integrity.
2. Ease of Separation:
   • Simplifies workflows by allowing trypsin removal post-digestion.
3. Enhanced Stability:
   • Maintains activity over longer periods due to resistance to denaturation.
4. Reduced Contamination:
   • Keeps samples free from protease contamination for clean downstream analysis.

Related Products

1. Trypsin, TPCK Treated:
   • Free form for non-immobilized applications.
2. Pierce Trypsin, MS Grade (Part No. 90057):
   • Optimized for mass spectrometry workflows.

Why Choose Immobilized TPCK Trypsin?

The Thermo Scientific Pierce Immobilized TPCK Trypsin is ideal for researchers seeking a robust, stable, and easily separable enzymatic tool for protein analysis and sequencing. Its immobilized format ensures precision, simplifies workflows, and enhances stability, making it an excellent choice for advanced proteomics and molecular biology applications.

Comparison Between Thermo Scientific™ Immobilized Trypsin, TPCK Treated and Trypsin, TPCK Treated

Key Advantages

Immobilized Trypsin, TPCK Treated

• Ease of Separation: Enzyme removal is straightforward post-digestion using centrifugation or filtration.
• Stability: Immobilization enhances stability and reduces heat-induced denaturation.
• Minimal Contamination: Keeps samples protease-free after digestion.
• Reusability: Resin can be reused, making it cost-effective for certain applications.

Trypsin, TPCK Treated

• Flexibility: Powder form allows solution-based reactions with no format restrictions.
• Versatility: Suitable for quick, single-use experiments where enzyme separation is not required.
• Simpler Preparation: Directly dissolvable in buffer for immediate use.

When to Choose Each

Conclusion

• Immobilized Trypsin, TPCK Treated is ideal for workflows requiring sample purity, reduced contamination, and reusability, especially in structural studies or affinity purification.
• Trypsin, TPCK Treated is preferred for versatile, solution-based applications where enzyme removal is not critical.

Your choice depends on whether you prioritize ease of separation and stability (Immobilized Trypsin) or flexibility and versatility (Trypsin in powder form).