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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Uracil DNA Glycosylase (UDG/UNG) is a high-purity enzyme designed to remove uracil residues from the sugar moiety of single- and double-stranded DNA. By catalyzing the cleavage of the N-glycosylic bond, UDG generates abasic sites without damaging the DNA backbone, preventing uracil-containing DNA from serving as a hybridization target or as a template for DNA polymerases.
This enzyme is an essential tool for PCR carry-over contamination prevention, significantly reducing false-positive results and ensuring reliable amplification outcomes. It is purified from E. coli expressing the E. coli ung gene and validated through exonuclease, endonuclease, and phosphatase quality assays.
Key Features
- Efficient Uracil Removal – Excises uracil residues from single- and double-stranded DNA.
- PCR Contamination Control – Prevents re-amplification of uracil-containing PCR products.
- High Specificity – Does not act on RNA.
- Validated Quality – Extensively tested for exonuclease, endonuclease, and phosphatase activity.
- Reliable Unit Definition – 1 unit releases 1 nmol of free uracil from ^3H-poly(dU) in 1 hour at 37 °C.
- Flexible Application – Suitable for cloning, PCR, qPCR, and molecular research workflows.
Applications
- Prevention of PCR carry-over contamination (reducing false positives)
- Cloning of PCR fragments
- Real-time PCR (qPCR) workflows
- DNA-based mutation studies and repair analysis
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Uracil DNA Glycosylase (UDG/UNG) |
| Catalog Number | 18054015 |
| Quantity | 100 units |
| Form | Liquid |
| Source | E. coli expressing the ung gene on a plasmid |
| Specificity | Removes uracil from DNA (no RNA activity) |
| Unit Definition | 1 U = release of 1 nmol uracil from ^3H-poly(dU) in 1 h at 37 °C |
| Reaction Conditions | 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl₂ |
| Compatible Applications | Standard PCR, qPCR |
| Shipping Condition | Wet or Dry Ice (approved) |
| Storage Conditions | –5 °C to –30 °C |
| Unit Size | Each |
Ordering Information
| Catalog Number | Quantity | Unit Size |
|---|---|---|
| 18054015 | 100 units | Each |
The Invitrogen™ Uracil DNA Glycosylase (UDG/UNG) is a reliable, high-quality enzyme for removing uracil from DNA, ensuring accurate and contamination-free PCR amplification. With proven specificity, rigorous quality validation, and compatibility with both standard PCR and qPCR, it is an essential reagent for molecular biology laboratories focused on cloning, mutation detection, and genetic analysis.