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Invitrogen™ DNase I, 20,000 U (50 to 375 U/μL)
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Enzymes | Forms | Freeze Dried |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ DNase I (Deoxyribonuclease I) is a high-activity endonuclease that digests single- and double-stranded DNA into oligodeoxyribonucleotides with 5′ phosphate termini. It has reduced ribonuclease (RNase) contamination to non-detectable levels, making it suitable for DNA removal from protein preparations, DNA nick translation, and fragment generation for sequencing.
This DNase I is purified from bovine pancreas and has a specific activity of 10,000–25,000 units/mg, ensuring efficient and reliable DNA degradation in molecular biology workflows.
Key Features & Benefits
1. High-Purity & High-Activity DNase I
- Efficiently digests DNA into oligonucleotides with 5′ phosphate ends.
- Reduced RNase contamination ensures minimal RNA degradation.
- Highly active enzyme (10,000–25,000 U/mg) for robust DNA digestion.
2. Versatile DNA Digestion Capabilities
- Cleaves both single- and double-stranded DNA for various applications.
- Generates randomly fragmented DNA, useful for sequencing and nick translation.
- Works in multiple reaction buffers, allowing compatibility with various assays.
3. Optimized for Protein & DNA Preparation
- Removes DNA contamination from protein extracts.
- Suitable for nick translation in DNA labeling experiments.
- Generates random DNA fragments for dideoxy sequencing and DNA footprinting.
Specifications
| Property | Details |
|---|---|
| Enzyme Type | DNase I (Deoxyribonuclease I) |
| Concentration | 50–375 U/μL |
| Specific Activity | 10,000–25,000 U/mg |
| Source | Purified from bovine pancreas |
| RNase Contamination | Reduced to non-detectable levels |
| Product Type | DNase I |
| Quantity | 20,000 Units |
| Shipping Condition | Wet Ice |
| Storage Conditions | -5°C to -30°C |
| Unit Size | Each |
Applications
1. Protein & RNA Preparation
- Removes DNA contamination from protein extracts.
- Used in chromatin and DNA-protein interaction studies.
- Suitable for protein purification workflows requiring DNA-free extracts.
2. DNA Processing & Sequencing
- Nick translation for DNA labeling and probe preparation.
- Generates random DNA fragments for dideoxy sequencing.
- Used in DNA footprinting assays for studying DNA-protein interactions.
3. Molecular Biology & Genetic Engineering
- Suitable for PCR preparation and DNA removal from samples.
- Used in restriction digestion and cloning workflows.
- Facilitates DNA degradation for functional genomics studies.
Pack Size & Catalog Number
| Pack Size | Catalog Number (SKU) |
|---|---|
| 20,000 Units | 18047019 |
Storage & Handling
- Store at -5°C to -30°C for long-term stability.
- Use sterile pipetting techniques to prevent contamination.
- Do not vortex—mix gently by inverting the tube.
Summary
Invitrogen™ DNase I is a high-purity, bovine pancreas-derived endonuclease optimized for DNA degradation in molecular biology, protein purification, and sequencing applications. Its low RNase contamination ensures reliable DNA digestion, making it suitable for DNA removal, nick translation, and DNA footprinting assays.