Invitrogen™ UltraPure™ Sucrose

Invitrogen™ UltraPure™ Sucrose is a molecular biology-grade sucrose powder designed for forming density gradients in a variety of separation applications. Its exceptional purity and reliability make it suitable for use in RNA separation, preparation of bacteriophage λ-DNA arms, and protein separation.

Key Features:

1. High Purity:
   • Molecular biology-grade sucrose ensures consistent and reliable results in sensitive applications.
2. Protease-Free:
   • Tested and certified to contain no detectable protease activity, protecting sensitive biological samples.
3. Versatile Applications:
   • Ideal for forming density gradients for separation of RNAs, DNA, and proteins.
4. Flexible Gradient Formation:
   • Supports gradients formed using gradient formers or manual layering of sucrose solutions.
5. Stable and Convenient:
   • Supplied as a stable powder that is easy to store and handle.

Specifications:

Applications:

1. Density Gradient Formation:
   • Forms precise gradients for separating RNA, DNA, and proteins via ultracentrifugation.
2. RNA Separation:
   • Used to isolate and purify RNA for downstream applications.
3. DNA Preparation:
   • Aids in the preparation of bacteriophage λ-DNA arms for molecular cloning.
4. Protein Separation:
   • Enables separation of proteins based on density gradients in biochemistry and molecular biology.

Advantages:

1. High Quality:
   • Molecular biology-grade sucrose ensures minimal contaminants and reliable experimental results.
2. Protease-Free:
   • Protects sensitive biological molecules from degradation during separation.
3. Convenient and Stable:
   • Easy-to-use powder format with long shelf life at room temperature.
4. Flexible Use:
   • Compatible with gradient formers or manual layering for customizable gradients.
5. Large Quantity:
   • Available in a 5 kg size for high-throughput and large-scale experiments.

Preparation Instructions:

1. Prepare Sucrose Solutions:
   • Dissolve the desired amount of UltraPure™ Sucrose in buffer or distilled water.
2. Create Gradient:
   • Form gradients using a gradient former or by layering solutions of varying concentrations into a centrifuge tube.
3. Centrifugation:
   • Process samples using ultracentrifugation according to experimental requirements.
4. Collect Fractions:
   • Use fraction collectors to isolate the desired components from the gradient.

Ordering Information:

Storage and Handling:

• Storage Conditions:
  • Store at room temperature in a dry environment.
• Shelf Life:
  • Refer to product label for expiration details.

Related Products:

• Invitrogen™ UltraPure™ DNase/RNase-Free Distilled Water: Ideal for preparing sucrose solutions.
• Invitrogen™ UltraPure™ Formamide: For RNA separation workflows.
• Invitrogen™ UltraPure™ Glycogen: A carrier for RNA and DNA precipitation.

Regulatory Notes:

• For Research Use Only: Not for use in diagnostic or therapeutic applications.

The Invitrogen™ UltraPure™ Sucrose provides a high-purity, reliable solution for density gradient formation and various molecular biology applications. Its protease-free composition and versatility make it a trusted choice for RNA, DNA, and protein separation workflows.