Gibco™ VP-SFM (1X)

Gibco™ VP-SFM (1X) is a serum-free, ultra-low protein medium formulated to support the growth of various mammalian cell lines, particularly for virus production and recombinant protein expression. This medium contains no proteins, peptides, or animal-origin components, ensuring a high level of purity and compatibility for research and manufacturing applications.

Key Features:

1. Serum-Free and Animal-Origin Free:
   • Designed to eliminate the variability associated with serum and animal-derived components.
2. Ultra-Low Protein:
   • Contains 5 μg/mL of protein, minimizing interference in downstream processes.
3. Optimized for Virus Production:
   • Ideal for VERO cells and other cell lines commonly used in viral vaccine production.
4. Broad Cell Line Compatibility:
   • Supports the growth of COS-7, MDCK, BHK-21 (suspension culture), and HEp2 cells.
5. Stable and Flexible Formulation:
   • Formulated without L-glutamine for enhanced stability. L-glutamine or GlutaMAX™-I supplement can be added prior to use.
6. Defined Composition:
   • Free from undefined components, ensuring reproducibility and suitability for regulated applications.

Specifications:

Applications:

1. Virus Production:
   • Optimized for large-scale virus production in VERO cells for vaccine research and manufacturing.
2. Recombinant Protein Expression:
   • Suitable for cell lines such as COS-7 and BHK-21 for producing therapeutic or research proteins.
3. Suspension Cell Culture:
   • Enables the growth of BHK-21 cells in suspension cultures, reducing reliance on anchorage-dependent systems.
4. General Mammalian Cell Culture:
   • Supports the growth and maintenance of other adherent and suspension cell lines, including HEp2 and MDCK.

Advantages:

1. High Purity:
   • No animal- or human-origin components reduce the risk of contamination and variability.
2. Reproducibility:
   • Chemically defined composition ensures batch-to-batch consistency.
3. Scalable and Versatile:
   • Suitable for small-scale research and large-scale biomanufacturing.
4. Flexible Usage:
   • Add L-glutamine or GlutaMAX™-I as required to customize the medium for specific applications.
5. Low Endotoxin Levels:
   • Ideal for sensitive applications like vaccine development and protein expression.

Protocol Overview:

1. Prepare Medium:
   • Add 20 mL of 200 mM L-glutamine or GlutaMAX™-I supplement per 1,000 mL of VP-SFM before use.
2. Inoculate Cells:
   • Seed cells into the prepared medium according to standard protocols.
3. Culture Conditions:
   • Incubate at 37°C with 5% CO₂ in a humidified atmosphere.
4. Monitor and Maintain:
   • Observe cell growth regularly and split cells as necessary.

Ordering Information:

Storage and Handling:

• Storage: Store at 2–8°C, away from light.
• Shelf Life: Refer to the product label for expiration details.

Related Products:

• Gibco™ GlutaMAX™-I Supplement: For stable L-glutamine addition.
• Gibco™ DPBS Without Calcium and Magnesium: For cell washing and preparation.
• Gibco™ MEM: For comparative cell culture studies.

Regulatory Notes:

• For Research Use Only: Not intended for diagnostic or therapeutic applications.
• Manufacturing-Grade: Suitable for further manufacturing and regulated workflows.

The Gibco™ VP-SFM (1X) is a versatile, high-performance medium that delivers reliable results in virus production, recombinant protein expression, and other advanced research applications. Its serum-free, animal origin-free formulation ensures safety, reproducibility, and regulatory compliance for your cell culture needs.