Invitrogen™ Dynabeads™ Untouched™ Human B Cells Kit, 10mL

The Invitrogen™ Dynabeads™ Untouched™ Human B Cells Kit is designed for the efficient and reliable isolation of pure, viable, and untouched B cells from peripheral blood mononuclear cells (PBMCs) through negative isolation. By depleting non-B cell populations such as T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes, this kit leaves untouched B cells free of beads or antibodies, ensuring high purity and functional integrity for downstream applications.

Key Features:

1. High Purity and Viability:
   • Isolates B cells with >97% viability and high recovery rates, ensuring functionality for downstream assays.
2. Negative Isolation Technology:
   • Depletes non-B cells using antibody-bound Dynabeads™, leaving B cells untouched and bead-free for optimal functionality.
3. Ease of Use:
   • Simple and scalable protocol with no need for columns or specialized equipment. Separation is achieved using a magnet.
4. Versatility:
   • Suitable for a wide range of applications, including flow cytometry, functional assays, molecular studies, and cell culture.
5. No Bead Contact:
   • Untouched B cells remain antibody- and bead-free, making them ideal for sensitive applications.

Specifications:

Kit Contents:

1. Depletion Dynabeads™:
   • 10 mL of MyOne™ SA Dynabeads™ for selective depletion of non-B cells.
2. Antibody Mix:
   • 2 mL of a biotinylated monoclonal antibody mix targeting non-B cells, including markers for:
     • CD2, CD14, CD16a/b, CD36, CD43, and CD235a (Glycophorin A).

Applications:

1. Flow Cytometry:
   • Analyze untouched B cells for phenotyping or cell surface marker expression.
2. Functional Assays:
   • Perform proliferation, cytokine production, or antibody secretion studies using isolated B cells.
3. Molecular Studies:
   • Use pure B cells for RNA or DNA extraction, gene expression profiling, and sequencing.
4. Cell Culture:
   • Cultivate isolated B cells for immunological research or co-culture experiments.
5. Immunotherapy Research:
   • Study B cell activation, signaling pathways, or their role in autoimmune diseases and cancer.

How It Works:

1. Antibody Labeling:
   • Add the biotinylated antibody mix to PBMCs. The antibodies bind to non-B cell populations.
2. Dynabead Binding:
   • Add MyOne™ SA Dynabeads™ to the sample. The beads bind to the antibody-labeled non-B cells.
3. Magnetic Separation:
   • Use a magnet to separate the bead-bound non-B cells from the untouched B cells.
4. Final Product:
   • The remaining cells are pure, viable, and untouched B cells, ready for analysis or downstream applications.

Key Benefits:

1. Untouched B Cells:
   • No direct bead or antibody contact ensures that the isolated B cells are in their native state for sensitive assays.
2. Simple and Fast Protocol:
   • Isolation can be completed in under an hour with minimal hands-on time.
3. Scalable:
   • Suitable for small-scale or large-scale cell isolation, processing up to 1 x 10⁹ cells per kit.
4. Compatible with Multiple Assays:
   • Ideal for flow cytometry, culture, and functional or molecular studies.

Storage and Handling:

• Store at 2°C to 8°C for optimal performance.
• Do not freeze the reagents, as this may affect the functionality of the antibody mix and beads.

For Research Use Only:

This product is intended for research purposes only and is not for use in diagnostic or therapeutic applications.