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Gibco™ MEM (Minimum Essential Medium), Liquid, 1X, 500 mL
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With
• L-glutamine
• Phenol Red
Without
• HEPES
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ MEM (Minimum Essential Medium) is one of the most widely used basal media for the growth and maintenance of a broad range of mammalian cell types. Originally developed by Harry Eagle, MEM is based on earlier formulations of Basal Medium Eagle (BME) and has become a foundational medium in cell culture research.
MEM supports both suspension and adherent cell lines and is commonly used in routine cell culture applications, including cancer research, virology, and primary cell studies. This formulation is prepared with Earle’s salts for use in CO₂ incubators and requires supplementation with serum or other growth factors.
Key Features
- Widely used basal medium for mammalian cell culture
- Suitable for a broad range of suspension and adherent cell types
- Formulated with Earle’s salts for CO₂-dependent buffering
- Contains L-glutamine and Phenol Red
- Does not contain HEPES or sodium pyruvate
- Sterile-filtered, ready-to-use liquid format
- Manufactured under cGMP-compliant and ISO 13485 standards
Cell Culture Applications
Gibco™ MEM is commonly used for culturing:
- HeLa
- BHK-21
- HEK293
- HEP-2
- HT-1080
- MCF-7
- Fibroblasts
- Primary rat astrocytes
MEM is frequently supplemented with 10% Fetal Bovine Serum (FBS) due to the absence of proteins, lipids, and growth factors.
Medium Characteristics
This MEM formulation includes:
With
- L-glutamine
- Phenol Red
Without
- HEPES
- Sodium pyruvate
MEM uses a sodium bicarbonate buffer system and requires a 5–10% CO₂ environment to maintain physiological pH.
Available Pack Size
| Catalog Number | Description | Quantity |
|---|---|---|
| 11095080 | Gibco™ MEM (Minimum Essential Medium), 1X Liquid | 500 mL |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | MEM (Minimum Essential Medium) |
| Concentration | 1X |
| Form | Liquid |
| Additives Included | Low glucose, L-glutamine, Phenol Red |
| Additives Excluded | No HEPES, No sodium pyruvate |
| Classification | Animal origin-free |
| Sterility | Sterile-filtered |
| Manufacturing Standard | cGMP-compliant, ISO 13485 |
| Shelf Life | 12 months |
| Quantity | 500 mL |
| Shipping Condition | Room temperature (ambient) |
| Storage Conditions | 2°C to 8°C (protect from light) |
| Unit Size | Each |
Storage & Handling
- Store at 2°C to 8°C, protected from light
- Ship under ambient conditions
- Supplement with serum (commonly 10% FBS) before use
- Maintain cultures in a 5–10% CO₂ incubator for proper pH buffering
Regulatory Status
For Research Use or Further Manufacturing Use Only.
Not for diagnostic use or direct administration into humans or animals.
Gibco™ MEM is a trusted and widely adopted basal medium for routine and specialty mammalian cell culture applications. With its proven formulation, CO₂-dependent buffering system, and compatibility with numerous cell lines, it remains an essential choice for laboratories performing reliable cell growth, maintenance, and research-based culture workflows.