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Invitrogen™ TMB Chromogen Solution (for ELISA), 500 mL
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Specifications:
| Application | Colorimetric Assay, ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Enzymes and Substrates | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₁₆H₂₀N₂ |
The Invitrogen™ TMB Chromogen Solution is a ready-to-use substrate reagent for horseradish peroxidase (HRP) detection in ELISA assays. Based on 3,3′,5,5′-tetramethylbenzidine (TMB), this chromogen undergoes a peroxidase-catalyzed reaction to produce a blue-colored product, measurable at OD 650 nm.
Designed for convenience, the solution requires no dilution or preparation, ensuring consistent, high-quality results for quantitative and qualitative immunoassays.
Key Features
- Ready-to-Use – No dilution or additional preparation required.
- HRP Substrate – Designed for horseradish peroxidase (HRP, POD)-based ELISAs.
- Colorimetric Detection – Produces a blue chromogenic signal measurable at OD 650 nm.
- Reliable & Consistent – High sensitivity and reproducibility for ELISA workflows.
- Convenient Storage – Stable at 2–8 °C; ships on wet ice.
Applications
- Enzyme-Linked Immunosorbent Assays (ELISAs)
- HRP-based immunoassays
- Quantitative and qualitative protein detection
- Antibody and antigen analysis
Technical Specifications
| Property | Details |
|---|---|
| Product Type | TMB Solution |
| Catalog Number | 002023 |
| Quantity | 500 mL |
| Substrate | 3,3′,5,5′-Tetramethylbenzidine (TMB) |
| Substrate Type | HRP (Horseradish Peroxidase) substrate |
| Detection Method | Colorimetric |
| Readout Wavelength | OD 650 nm (blue color) |
| Form | Ready-to-use solution |
| Storage Conditions | 2–8 °C |
| Shipping Conditions | Wet ice |
| Unit Size | Each |
Ordering Information
| Catalog Number | Quantity | Unit Size |
|---|---|---|
| 002023 | 500 mL | Each |
The Invitrogen™ TMB Chromogen Solution (for ELISA) provides a simple, reliable, and high-quality substrate for HRP-based assays. With its ready-to-use formulation, colorimetric readout, and robust reproducibility, it is an essential reagent for ELISA-based protein, antibody, and antigen detection workflows in research laboratories.