Invitrogen™ eBioscience™ 1X RBC Lysis Buffer, 200mL

The Invitrogen™ eBioscience™ 1X RBC Lysis Buffer is a pre-diluted, ready-to-use solution specifically formulated for the effective lysis of erythrocytes in single-cell suspensions from mouse hematopoietic tissues (e.g., spleen) and human peripheral blood. The buffer contains ammonium chloride, which selectively lyses non-nucleated red blood cells while leaving leukocytes and lymphocytes intact, preserving cell viability and integrity for downstream applications.

Key Features and Benefits

• Pre-diluted, Ready-to-Use
  Convenient 1X liquid format eliminates the need for dilution or buffer preparation.
• Selective Red Cell Lysis
  Efficiently removes erythrocytes while sparing nucleated white blood cells in single-cell suspensions.
• Gentle on Immune Cells
  Preserves lymphocyte viability and surface marker expression—ideal for flow cytometry and cell culture.
• Reliable Performance
  Widely used in immunology and hematology workflows for cleaner cell preps and more accurate downstream analysis.

Applications

• Flow Cytometric Analysis
• Immune cell isolation from mouse spleen or human blood
• Tissue dissociation for single-cell analysis
• Pre-treatment of samples before staining, sorting, or culture

Not effective for lysing nucleated red blood cells (e.g., from fetal liver or cord blood).

Technical Specifications

Invitrogen™ eBioscience™ 1X RBC Lysis Buffer offers a fast, reliable, and gentle method for eliminating red blood cells from mouse or human samples, enabling clearer and more accurate downstream analysis in flow cytometry, cell culture, and immunological research. Its ready-to-use format and consistent performance make it a staple in hematology and immunology workflows.