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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence)
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Reading Options: Top and Bottom
No. of dispensers: 0
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TGD0) is an advanced, fully modular, and upgradable instrument designed for high-sensitivity multimode detection across Absorbance, Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF).
Equipped with a built-in Gas Control Module, it allows precise regulation of CO₂ (0.1–15%) and O₂ (1–21%) concentrations—making it ideal for cell-based and live-cell assays. The instrument delivers exceptional flexibility and precision for enzyme kinetics, FRET/TR-FRET assays, DNA/RNA quantification, and luminescent cellular analyses.
With its automatic dynamic range selection, smart safety controls, and self-calibrating optical system, the Varioskan LUX sets a new standard for multimode plate readers, combining usability, accuracy, and reproducibility for high-performance research applications.
Key Features and Benefits
1. Four Integrated Detection Technologies
- Supports Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity (incl. FRET), Luminescence, and Time-Resolved Fluorescence (TRF/TR-FRET).
- Top and bottom reading optics provide maximum assay flexibility for both homogeneous and adherent cell-based formats.
- Dual monochromator system ensures precise wavelength control for excitation and emission measurements.
- Optional expansion for AlphaScreen™ and AlphaLISA™ technologies.
2. Built-In Gas Control Module
- Regulates CO₂ (0.1–15%) and O₂ (1–21%) for physiological cell-based assay conditions.
- Enables accurate environmental control for live-cell kinetic measurements.
- Perfect for applications in cell signaling, metabolism, and viability assays.
3. High Sensitivity and Broad Dynamic Range
- Fluorescence Sensitivity: <0.4 fmol fluorescein/well (top read).
- Time-Resolved Fluorescence Sensitivity: <1 amol/well (white 384-well plate).
- Luminescence Sensitivity: <7 amol ATP/well.
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Dynamic Range:
- Fluorescence: >6 decades (top read).
- Luminescence: >7 decades.
- TRF: >6 decades.
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
4. Intelligent Automation and Workflow Control
- Automatic dynamic range selection per well for optimized sensitivity.
- Smart Safety Controls detect and alert users to potential errors before runs.
- Self-calibration and diagnostics ensure long-term performance stability.
- Five measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Orbital shaking and temperature regulation (ambient +4°C to 45°C) maintain assay consistency.
5. Integrated SkanIt™ Software
- SkanIt™ Research Edition included for complete control and analysis.
- License-free installation for multi-user operation.
- Virtual Pipetting Tool simplifies assay setup and sample-to-well mapping.
- Real-time spectra and kinetic visualization during measurements.
- Automatic data saving prevents data loss from interruptions.
- Built-in data analysis tools: Blank subtraction, standard curve, dose-response, spectral fitting, kinetic modeling, and custom formulas.
- Export flexibility: *.xlsx, *.pdf, *.xml, *.txt, and auto-email functionality.
6. Certified and Validated Performance
- HTRF™ certified by Cisbio Bioassays.
- DLReady™ validated by Promega Corporation.
- Compliant with regulatory data standards; 21 CFR Part 11 support available via Drug Discovery Edition.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top and Bottom |
| Gas Control Module | CO₂: 0.1–15%; O₂: 1–21% |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF Excitation: 334 nm; TRF Emission: 400–700 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI: >6 decades; TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI: <0.4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | SkanIt™ Research Edition |
Applications
- Cell-Based Assays: Reporter gene, cell proliferation, cytotoxicity, and metabolic studies under controlled gas environments.
- Molecular Biology: DNA, RNA, and protein quantification via absorbance and fluorescence.
- Enzyme Kinetics: Real-time enzyme activity and inhibition analysis.
- Fluorescence and TRF Assays: FRET, TR-FRET/hTRF, and HTRF-based studies.
- Luminescent Assays: ATP, luciferase, and cell signaling detection.
- Spectral Scanning: Wavelength optimization and spectral profiling for assay development.
- Drug Discovery: Quantitative fluorescence and luminescence-based compound screening.
Unique Advantages
- Combines four detection technologies for comprehensive assay flexibility.
- Integrated Gas Control Module for live-cell and physiological assay conditions.
- TRF and TR-FRET capability for time-resolved fluorescence applications.
- Automatic calibration and dynamic range optimization ensure reproducibility.
- HTRF™ and DLReady™ certifications guarantee validated performance.
- Modular, upgradable design for future expansion (AlphaScreen™, TRF, and more).
- SkanIt™ Software delivers intuitive workflow management and traceable data integrity.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TGD0) offers unparalleled versatility in multimode microplate detection, integrating absorbance, fluorescence, luminescence, and time-resolved fluorescence within a compact, intelligent system.
Its built-in Gas Control Module, high sensitivity, and smart automation make it ideal for advanced cell-based, enzymatic, and fluorescence energy transfer assays.
Combined with SkanIt™ Software, the system provides reliable, traceable, and high-quality data, empowering laboratories to achieve superior accuracy and workflow efficiency.
A preferred choice for research laboratories, pharmaceutical developers, and molecular biologists, the Varioskan LUX combines cutting-edge optics, environmental control, and automation to deliver excellence in multimode assay performance.