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Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment is a highly purified recombinant enzyme derived from E. coli, engineered to lack the 5′→3′ exonuclease activity of intact DNA Polymerase I, while retaining both 5′→3′ polymerase activity and 3′→5′ exonuclease (proofreading) activity. This enzymatic profile makes it ideal for precise DNA manipulations in molecular biology workflows where removal of the 5′→3′ exonuclease function is critical.
This Klenow fragment is extensively tested for purity and activity and is shipped with a 10X REact™ 2 Buffer for optimal performance.
Applications
-
Fill-in of 5′ Overhangs
Used to fill recessed 3′ ends or convert 5′ overhangs into blunt ends in double-stranded DNA. -
Random Primer Labeling
Facilitates synthesis of radiolabeled or fluorescent probes via random priming techniques. -
DNA Sequencing
Applicable for sequencing of both single-stranded and double-stranded DNA templates. -
Site-Directed Mutagenesis
Supports generation of point mutations by incorporating specific nucleotide changes in vitro.
Key Features and Benefits
- Lacks 5′→3′ Exonuclease Activity: Prevents degradation of DNA strands during reactions.
- Retains 5′→3′ Polymerase and 3′→5′ Exonuclease Functions: Enables DNA synthesis and proofreading.
- High Purity and Performance: Validated using SDS-PAGE and functional assays.
- Ready-to-use Formulation: Supplied with REact™ 2 Buffer for optimal enzymatic conditions.
- Flexible Reaction Setup: Compatible with standard cloning and labeling workflows.
Specifications
| Parameter | Detail |
|---|---|
| Enzyme Type | DNA Polymerase I (Klenow Fragment) |
| Exonuclease Activity | 3′→5′ only (proofreading) |
| 5′→3′ Exonuclease Activity | Absent |
| Polymerase Activity | 5′→3′ DNA synthesis |
| Hot Start | No |
| Source | Recombinant E. coli |
| Unit Sizes Available | 100, 500, and 1000 Units (2×500) |
| Supplied Buffers | 10X REact™ 2 Buffer, dilution buffer |
| Storage | –20 °C |
| Shipping | Wet or Dry Ice |
| Format | Liquid enzyme with buffer |
| Applications | DNA labeling, blunt-ending, mutagenesis, sequencing |
Contents and Storage
-
Supplied with:
-
10X REact™ 2 Buffer:
[500 mM Tris-HCl (pH 8.0), 100 mM MgCl₂, 500 mM NaCl] - Dilution buffer
-
10X REact™ 2 Buffer:
- Storage: Store at –20 °C. Avoid repeated freeze-thaw cycles.
The Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment is a versatile and essential tool for molecular biology workflows that require DNA synthesis without strand degradation. Its balanced enzymatic profile makes it ideal for precise DNA modifications, blunt-end repair, probe generation, and mutagenesis. Backed by Thermo Fisher Scientific’s rigorous quality standards, this enzyme delivers consistent, high-performance results for research and genomic applications.
T4 DNA Polymerase Brochure
User Guide
Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment is a highly purified recombinant enzyme derived from E. coli, engineered to lack the 5′→3′ exonuclease activity of intact DNA Polymerase I, while retaining both 5′→3′ polymerase activity and 3′→5′ exonuclease (proofreading) activity. This enzymatic profile makes it ideal for precise DNA manipulations in molecular biology workflows where removal of the 5′→3′ exonuclease function is critical.
This Klenow fragment is extensively tested for purity and activity and is shipped with a 10X REact™ 2 Buffer for optimal performance.
Applications
-
Fill-in of 5′ Overhangs
Used to fill recessed 3′ ends or convert 5′ overhangs into blunt ends in double-stranded DNA. -
Random Primer Labeling
Facilitates synthesis of radiolabeled or fluorescent probes via random priming techniques. -
DNA Sequencing
Applicable for sequencing of both single-stranded and double-stranded DNA templates. -
Site-Directed Mutagenesis
Supports generation of point mutations by incorporating specific nucleotide changes in vitro.
Key Features and Benefits
- Lacks 5′→3′ Exonuclease Activity: Prevents degradation of DNA strands during reactions.
- Retains 5′→3′ Polymerase and 3′→5′ Exonuclease Functions: Enables DNA synthesis and proofreading.
- High Purity and Performance: Validated using SDS-PAGE and functional assays.
- Ready-to-use Formulation: Supplied with REact™ 2 Buffer for optimal enzymatic conditions.
- Flexible Reaction Setup: Compatible with standard cloning and labeling workflows.
Specifications
| Parameter | Detail |
|---|---|
| Enzyme Type | DNA Polymerase I (Klenow Fragment) |
| Exonuclease Activity | 3′→5′ only (proofreading) |
| 5′→3′ Exonuclease Activity | Absent |
| Polymerase Activity | 5′→3′ DNA synthesis |
| Hot Start | No |
| Source | Recombinant E. coli |
| Unit Sizes Available | 100, 500, and 1000 Units (2×500) |
| Supplied Buffers | 10X REact™ 2 Buffer, dilution buffer |
| Storage | –20 °C |
| Shipping | Wet or Dry Ice |
| Format | Liquid enzyme with buffer |
| Applications | DNA labeling, blunt-ending, mutagenesis, sequencing |
Contents and Storage
-
Supplied with:
-
10X REact™ 2 Buffer:
[500 mM Tris-HCl (pH 8.0), 100 mM MgCl₂, 500 mM NaCl] - Dilution buffer
-
10X REact™ 2 Buffer:
- Storage: Store at –20 °C. Avoid repeated freeze-thaw cycles.
The Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment is a versatile and essential tool for molecular biology workflows that require DNA synthesis without strand degradation. Its balanced enzymatic profile makes it ideal for precise DNA modifications, blunt-end repair, probe generation, and mutagenesis. Backed by Thermo Fisher Scientific’s rigorous quality standards, this enzyme delivers consistent, high-performance results for research and genomic applications.
T4 DNA Polymerase Brochure
User Guide
Spécifications
| Pack Size | 500 UNITS, 100 UNITS, 2 x 500 UNITS |