Varioskan™ ALF Multimode Microplate Reader, for 6- to 384-well microplates

The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays. Its photometric optical design is based on a xenon flash lamp and monochromator, enabling continuous wavelength selection from UV through visible range up to 1000 nm. The broad wavelength range enables many common assays, including nucleic acid and protein quantification, bacterial growth curves, ELISA, and cell viability.

Enhance your fluorescence and luminescence measurements with the filter-based optics

With the Varioskan ALF reader, fluorescence and luminescence measurements are performed with filter-based optics for optimal specificity and sensitivity. The system comes with three pre-installed excitation and emission filter sets suitable for many common fluorometric applications like Quant-IT DNA quantitation assays and PrestoBlue and AlamarBlue cell viability assays. Additionally, five open filter positions are available for customized fluorometric and luminometric measurements. The filter slots are easy to access on the instrument’s side with no tools required for filter exchange. Also, commonly used FRET and BRET assays are easy to set up and perform thanks to Varioskan ALF reader’s unique filter-based optics design.

Achieve consistently accurate results with automatic gain adjustment

The automatic gain adjustment feature selects the ideal fluorometric or luminometric reading range for your instrument based on signal intensity in the well, eliminating the need to manually adjust measurement parameters. The results are consistent no matter what signals are measured.

Additional features

• Kinetic and endpoint measurements for a variety of applications

• Spectral scanning for photometric measurements

• Separate optimized measurement modes for absorbance and turbidimetric measurements

• A self-diagnostics system that includes a set of initialization tests and adjustments, ensuring instrument readiness for operation

• Compatible with 6- to 384-well microplates with and without lids

• Reads µDrop and µDrop Duo plates for microvolume analysis of DNA, RNA, and proteins

• Performs onboard shaking and incubation for temperature-critical assays

• Operated through SkanIt software

   --Unlimited installation on multiple computers, no annual fee required

   --Includes an extensive cloud-based library of ready-made protocols

   --Two editions available: Research Edition for life sciences and Drug Discovery Edition with features for compliance with 21 CFR Part 11

   --Multiple user interface languages available

   --Automation compatibility for integration with automation interface

• Thermo Scientific verification plates provide comprehensive and traceable validation of the instrument optical performance. Ready-made SkanIt software protocols provide easy to read reports with defined measurement parameter limits.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Product Type: Multimode Microplate Reader
• Cuvettes: No
• Detector Type: Photomultiplier Tube (PMT)
• Incubator Temperature: 45°C
• Interface: PC Control, PC Software (Thermo Scientific SkanIt Software)
• Light Source: Xenon Flash Lamp

Measurement Speed; 

1. Photometry: ≤10 sec. (fast mode); 
2. Fluorometry and Luminometry: ≤20 sec. (96-well plate)

• Product Line: Varioskan
• Reading Options: Endpoint, Kinetic
• Software Type: SkanIt Software
• Spectral Scanning Speed: ≤16 sec. (Fast mode)
• Absorbance Range: 200 to 1000 nm
• Accuracy (Photometric): ±1.0% + 0.003 Abs (0 to 2 Abs); ±2.0% (2.0 to 2.5 Abs) @ 450 nm
• Depth (Metric): 61 cm
• Dynamic Range: ≥6 Decades
• Emission Wavelength Range: 210/720 nm
• Excitation Wavelength Range: 200/710 nm
• For Use With (Application) Photometric, fluorometric and luminometric microplate assays
• Height (Metric) 40 cm
• Linearity: 0 to 2.5 Abs, 2.0% @ 450 nm
• Precision: SD <0.003 Abs, or CV <1.0% @ 450 nm
• Voltage: 100/115/220/230 V
• Warranty: Standard
• Width (Metric): 34 cm
• Unit Size: 1 each