Toluene-α-sulphonyl fluoride 99%

Apollo Scientific PMSF (Toluene-α-sulphonyl fluoride) is a potent protease inhibitor widely used in biochemical and molecular biology research. PMSF irreversibly inhibits serine proteases and some cysteine proteases, making it essential for studies requiring enzyme activity suppression.

Key Features:

1. Specific Protease Targeting:
   • Effectively inhibits serine proteases like trypsin, chymotrypsin, and thrombin.
   • Also inhibits cysteine proteases such as papain (reversible with DTT).
2. Effective at Low Concentrations:
   • Active at final concentrations of 0.1 to 1 mM in most protein solutions.
3. Versatile Solubility:
   • Due to limited water solubility, PMSF should be dissolved in solvents like ethanol, methanol, or isopropanol before being added to aqueous buffers.
4. Additional Applications:
   • Can inhibit mammalian acetylcholinesterase, broadening its utility in enzyme studies.

Applications:

1. Protease Inhibition:
   • Prevents unwanted protease activity during protein extraction and purification.
2. Enzyme Activity Studies:
   • Ideal for assays requiring suppression of proteolytic enzymes.
3. Sample Preparation:
   • Protects proteins from degradation in cell lysates or other biological samples.

Specifications:

Advantages:

Handling and Storage:

• Storage Conditions: Store in a cool, dry place away from moisture and light.
• Safety Precautions:
  • Hazard Classification: PMSF is toxic and an irritant; handle with care.
  • Wear appropriate protective equipment, including gloves and safety glasses.

Chemical and Physical Properties:

Apollo Scientific PMSF is an essential reagent for biochemical workflows requiring protease inhibition, offering reliable performance and broad application utility.