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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ SalI restriction enzyme is a high-quality endonuclease that recognizes the palindromic sequence 5′ G^TCGAC 3′ / 3′ CAGCT^G 5′ and generates 5′ overhangs. Optimized to perform at 37 °C in Buffer O, it is part of Thermo Scientific’s extensive portfolio of conventional restriction enzymes designed for precision in molecular cloning and genetic analysis workflows.
Unlike FastDigest™ enzymes, conventional Thermo Scientific enzymes are optimized for the Five Buffer System and supplied with the universal Tango buffer for efficient double digestions. Each lot undergoes stringent quality control to ensure consistent activity, reproducibility, and absence of contaminating nucleases.
Key Features
- Reliable performance – optimized for complete digestion under recommended conditions.
- Superior quality assurance – manufactured under stringent QC standards.
- Five Buffer System – convenient, color-coded buffers for flexibility across enzymes.
- Universal Tango buffer – ensures compatibility and efficiency in double digestions.
- Stabilized reaction conditions – premixed BSA included in buffers enhances enzyme stability and sequesters contaminants.
- Wide compatibility – available in multiple formats to support various cloning scales.
Applications
- Molecular cloning and recombinant DNA workflows.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP) studies.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | SalI |
| Recognition Sequence | 5′ G^TCGAC 3′ / 3′ CAGCT^G 5′ |
| Cleavage Product | 5′ protruding ends |
| Concentration | 10 U/µL |
| Optimal Reaction Temperature | 37 °C |
| Compatible Buffer | 10X Buffer O (Five Buffer System) |
| Supplied Buffer | 10X Buffer O + Universal Tango buffer |
| Methylation Sensitivity | Sensitive to CpG methylation; not Dam/Dcm methylation-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage | –20 °C |
Available Formats
| Catalog Number | Quantity | Unit Size | Format |
|---|---|---|---|
| ER0641 | 1,500 units | 10 U/µL | Enzyme + Buffers |
| ER0642 | 5 × 1,500 units | 10 U/µL | Enzyme + Buffers |
| ER0645 | 2,000 units | 10 U/µL | Enzyme + Buffers |
Thermo Scientific™ SalI (10 U/µL) delivers consistent, high-fidelity DNA cleavage for traditional cloning applications. With its robust quality control, convenient Five Buffer System, and compatibility with universal Tango buffer, it supports single and double digestions with ease. Designed for research requiring precision and reproducibility, SalI remains a reliable choice for molecular biology laboratories engaged in cloning, genotyping, and nucleic acid analysis.
- Pack Size: 2,000 UNITS 5 x 1,500 units 1,500 Units