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Thermo Scientific™ RIPA Lysis and Extraction Buffer
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Specifications:
| Application | Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use reagent for the effective lysis and extraction of cytoplasmic, membrane, and nuclear proteins from cultured mammalian cells. Based on the classic Radio-Immunoprecipitation Assay (RIPA) formulation, this buffer offers a robust and versatile solution for protein analysis workflows, including Western blotting, reporter assays, immunoassays, and protein purification.
This buffer contains a fully disclosed composition of ionic and non-ionic detergents, providing transparency and compatibility assurance for downstream applications. It is designed for use with plated adherent cells or pelleted suspension cells and can be further enhanced with protease and phosphatase inhibitors (sold separately).
Key Features and Benefits
- Ready-to-use formulation – eliminates time-consuming buffer preparation
- Disclosed composition – full transparency for compatibility and optimization
- Versatile protein extraction – lyses cytoplasmic, membrane, and nuclear fractions
- Application-flexible – ideal for immunoblotting, ELISA, protein assays, and more
- Compatible with protease and phosphatase inhibitors such as Halt™ Inhibitor Cocktails
- Supplied in 100 mL and 250 mL volumes to suit varied throughput needs
Applications
- Protein extraction from mammalian cells (adherent and suspension)
- Western blotting (SDS-PAGE sample preparation)
- ELISA and other immunoassays
- Reporter gene assays
- Protein quantification (e.g., BCA assay)
- Phosphorylation studies and pathway analysis
- Sample preparation for co-immunoprecipitation and pull-down assays
Specifications
| Attribute | Description |
|---|---|
| Product Type | Extraction buffer (RIPA) |
| Format | Liquid, ready-to-use |
| Volume | 100 mL (89900) or 250 mL (89901) |
| Buffer Compatibility | Protease & phosphatase inhibitors optional |
| Target Sample | Cultured mammalian cells |
| Target Fractions | Cytoplasmic, nuclear, and membrane proteins |
| Detergents Included | Ionic and non-ionic |
| Storage Temperature | 4 °C |
| Intended Use | Research use only (RUO) |
Buffer Composition Overview
While the exact proprietary formulation is not provided here, typical RIPA buffer includes:
- Tris-HCl (buffering agent)
- NaCl (ionic strength)
- NP-40 or Triton X-100 (non-ionic detergent)
- SDS and sodium deoxycholate (ionic detergents)
- Optional additives: EDTA, protease inhibitors, phosphatase inhibitors
The Thermo Scientific™ RIPA Lysis and Extraction Buffer is a proven solution for reliable, multi-fraction protein extraction across diverse research applications. Its transparency, ease of use, and compatibility with commonly used inhibitors and detection methods make it an essential component of any molecular biology or protein research lab.
- Pack Size: 100 mL 250 mL