Thermo Scientific™ PVDF Transfer Membranes, 0.45 μm

Thermo Scientific™ Pierce™ PVDF (Polyvinylidene Difluoride) Transfer Membranes are designed for high-sensitivity protein and nucleic acid blotting applications. These membranes feature a 0.45 μm pore size, making them ideal for the electrotransfer of proteins larger than 10 kDa, and they are well suited for Western blotting, Southern and Northern blotting, and dot/slot blot applications.

Manufactured from high-grade PVDF, these membranes offer a high binding capacity, mechanical durability, and chemical resistance, making them particularly effective for multiple reprobing cycles and compatible with various detection methods including chemiluminescence, fluorescence, and radiolabeling.

Key Features and Benefits

• High binding capacity
  • Goat IgG: ~294 µg/cm²
  • BSA: ~215 µg/cm²
  • Insulin: ~160 µg/cm²
• Pore size: 0.45 µm – optimal for transfer of proteins >10 kDa
• Excellent mechanical strength – ideal for repeated stripping and reprobing
• Durable and solvent-compatible – resistant to organic solvents, acids, and mild bases
• Flexible format options – choose pre-cut sheets for convenience or rolls for customizable sizes
• Compatible with all major transfer systems – wet, semi-dry, or rapid dry transfer
• Pre-activation required – hydrate in 100% methanol before use

Specifications

Applications

• Western blotting – including chemiluminescent and fluorescent detection
• Southern and Northern blotting – high-efficiency nucleic acid transfer
• Protein or nucleic acid dot/slot blotting
• Quantitative and qualitative immunodetection
• Stripping and reprobing protocols

Pierce™ PVDF Transfer Membranes (0.45 μm) are the gold standard for laboratories requiring robust, high-sensitivity transfer of biomolecules in a range of blotting workflows. Choose the pre-cut format for ease of use in standard mini-gel setups or the roll format for flexibility across blot sizes and formats.