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Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
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Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | –80°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive, multiplex bioluminescence assay that enables simultaneous detection of Green Renilla and Red Firefly luciferase activity in mammalian whole-cell lysates. This dual-reporter format allows researchers to measure two distinct biological activities in the same sample, eliminating the need for sequential reagent addition and signal quenching required in traditional dual-luciferase assays.
With spectrally resolvable emissions (Renilla: λmax ≈ 535 nm, Firefly: λmax ≈ 613 nm), the kit supports filter-based wavelength separation for accurate and independent quantification of both reporters.
Key Features
- Simultaneous Dual Detection – Concurrent measurement of Green Renilla and Red Firefly luciferases in one assay.
- High Sensitivity – Detects low reporter expression levels with minimal background.
- No Quenching Required – Single-step reagent addition simplifies workflow.
- Multiplex Capability – Study two regulatory elements, pathways, or targets simultaneously.
- Fast & Automation-Friendly – Compatible with high-throughput screening in 96- and 384-well formats.
Applications
- Promoter & Regulatory Element Studies – Analyze transcriptional activity of two elements in parallel.
- Signal Pathway Analysis – Monitor two pathways within the same cell population.
- Off-Target Effect Screening – Evaluate unintended activity of gene modulators.
- Drug Discovery & Screening – Test compounds against multiple targets in one assay.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Bioluminescence |
| Reporters | Green Renilla luciferase, Red Firefly luciferase |
| Emission Peaks | Renilla: ~535 nm; Firefly: ~613 nm |
| Substrates | Coelenterazine (Renilla), D-Luciferin (Firefly) |
| Assay Format | Homogeneous, single-step |
| Compatible Formats | 96-well and 384-well microplates |
| Cell Types | Mammalian |
| Storage | –80°C (kit), component-specific storage per datasheet |
| Kit Sizes | 100 reactions (Cat. No. 16185), 1000 reactions (Cat. No. 16186) |
Kit Contents
- Renilla-Firefly Dual Assay Buffer – 5 mL (store at 4°C)
- 100X Coelenterazine – 50 μL (store at –80°C)
- D-Luciferin – 3 mg (store at 4°C)
- Luciferase Cell Lysis Buffer (2X) – 6 mL (store at RT)
The Pierce™ Renilla-Firefly Luciferase Dual Assay Kit offers a streamlined, sensitive, and multiplex-capable solution for researchers who need simultaneous dual-reporter analysis in mammalian cells. Its single-step protocol reduces variability, increases throughput, and supports high-content analysis for gene expression, pathway studies, and drug discovery.
- Pack Size: For 100 Assays For 1000 Assays