Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0

The Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding Buffer (pH 8.0) is specifically designed for antibody purification workflows where preservation of native protein structure and function is critical. It is a phosphate-free, ready-to-use binding buffer optimized for use with Protein A and Protein A/G affinity chromatography resins in conjunction with Gentle Ag/Ab Elution Buffer (pH 6.6).

This buffer enables efficient capture of antibodies under physiological conditions and is ideal for sensitive antigen-antibody interactions that may be disrupted under acidic elution conditions. The formulation includes 0.05% sodium azide for microbial stability and ensures high binding efficiency without precipitation issues often associated with phosphate buffers.

Key Features & Benefits

• Phosphate-Free Formulation
  Prevents precipitation when used with high-salt, near-neutral elution buffers, ensuring system compatibility and buffer clarity.
• Maintains Native Protein Structure
  Suitable for non-denaturing antibody-antigen interactions, preserving biological activity of both target and antibody.
• Ready-to-Use 1X Format
  Preformulated and filtered for immediate use—no dilution or adjustment necessary.
• Optimized for Protein A and A/G Affinity Purification
  Ideal for use with Thermo Scientific™ Protein A and A/G resins in antibody capture protocols.
• Compatible with High Salt Elution Conditions
  Enables effective pairing with Pierce™ Gentle Ag/Ab Elution Buffer (pH 6.6) for mild, effective elution.

Applications

• Non-denaturing antibody purification from serum, ascites, or hybridoma supernatants
• Affinity purification workflows using Protein A or Protein A/G
• Preparation of antibodies for immunoassays, immunoprecipitation (IP), or functional studies

Product Specifications

Related Products

• Pierce™ Gentle Ag/Ab Elution Buffer, pH 6.6 (Cat. No. 21027)
• Pierce™ Protein A Agarose Resins and Spin Columns
• Pierce™ Antibody Purification Kits and Desalting Columns

The Thermo Scientific™ Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0, is a high-quality, phosphate-free solution specifically engineered for sensitive antibody purification applications. Its compatibility with non-acidic elution conditions ensures the integrity of both antibodies and antigens during purification—making it an ideal choice for research workflows requiring gentle and reproducible separation of immune complexes.