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Thermo Scientific™ N-Fmoc-S-trityl-L-cysteine, 95%, HPLC
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Specifications:
| Application | Protein Biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Amino Acid & Biochemicals | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | HPLC Grade | Formula | C₃₇H₃₁NO₄S |
Thermo Scientific™ N-Fmoc-S-Trityl-L-Cysteine is a protected cysteine derivative used in solid-phase peptide synthesis (SPPS), proteomics research, protein engineering, bioconjugation, and pharmaceutical development. It features Fmoc protection on the α-amino group and trityl protection on the cysteine thiol side chain, enabling controlled peptide elongation while helping prevent premature oxidation or unwanted thiol side reactions.
Cysteine is an important amino acid in peptide and protein chemistry due to its role in disulfide bond formation, protein folding, enzyme activity, and site-specific modification. This reagent is therefore widely used for synthesizing cysteine-containing peptides, cyclic peptides, disulfide-rich peptides, and cysteine-modified biomolecules.
This product was originally part of the Alfa Aesar portfolio and is now offered under Thermo Scientific Chemicals.
Key Features
| Feature | Details |
|---|---|
| High Purity | 95% grade; HPLC assay ≥94.0% |
| Fmoc Protection | Protects the α-amino group for controlled peptide synthesis |
| Trityl Thiol Protection | Protects the cysteine thiol group from premature oxidation |
| SPPS Compatible | Suitable for Fmoc-based solid-phase peptide synthesis |
| Cysteine Building Block | Enables incorporation of protected cysteine into synthetic peptides |
| Research Versatility | Useful in proteomics, protein engineering, drug discovery, and bioconjugation |
Chemical Properties & Identifiers
| Property | Value |
|---|---|
| Product Name | Thermo Scientific™ N-Fmoc-S-Trityl-L-Cysteine, 95% |
| Synonym | Fmoc-Cys(Trt)-OH |
| CAS Number | 103213-32-7 |
| IUPAC Name | (2S)-2-(9H-fluoren-9-ylmethoxycarbonylamino)-3-(tritylsulfanyl)propanoic acid |
| Molecular Formula | C₃₇H₃₁NO₄S |
| Molecular Weight | 585.71 g/mol |
| Appearance | White to pale cream powder |
| Optical Rotation | +15° to +19°; c = 1 in THF |
| Melting Point | 174.0–182.0 °C |
| Assay by HPLC | ≥94.0% |
| Assay by Aqueous Acid-Base Titration | ≥94.0% to ≤106.0% |
| Solubility | Insoluble in water; soluble in most organic solvents |
| Functional Groups | Fmoc, trityl-protected thiol, carboxylic acid |
Applications
Thermo Scientific™ N-Fmoc-S-Trityl-L-Cysteine is suitable for:
- Fmoc-based solid-phase peptide synthesis
- Incorporation of cysteine residues into synthetic peptides
- Cysteine-containing peptide synthesis
- Disulfide bond-containing peptide preparation
- Cyclic peptide synthesis
- Proteomics and biochemical research
- Protein engineering and structural biology studies
- Site-specific cysteine modification
- Bioconjugation and thiol-functionalized peptide chemistry
- Pharmaceutical peptide and drug discovery research
- Enzyme activity and inhibition studies
- Bioorganic chemistry and custom biomolecule synthesis
Ordering Information
| Catalog Number | Quantity |
|---|---|
| H27363.06 | 5 g |
| H27363.14 | 25 g |
Storage & Handling
Store in a cool, dry place in a tightly sealed container. Keep away from oxidizing agents, moisture, and extreme temperatures. Handle in a well-ventilated laboratory environment using appropriate personal protective equipment, including gloves, lab coat, and safety goggles.
Research Use Statement
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
- Pack Size: 5g 25g