Thermo Scientific™ Invitrogen™ SlowFade™ Gold Antifade Mountant

Invitrogen™ SlowFade™ Gold Antifade Mountant is a glycerol-based liquid mounting medium designed to protect fluorescent dyes from photobleaching during fluorescence microscopy. It is optimized for immediate use with fluorescently labeled cell or tissue samples mounted on microscope slides. The mountant ensures long-term stability of fluorescence signals, making it ideal for applications involving fluorescence imaging. SlowFade Gold Antifade Mountant is available in multiple formats with or without DAPI for nuclear staining.

Key Features:

1. Photobleaching Protection: Prevents fading of fluorescent dyes, ensuring reliable and reproducible imaging results.
2. Ready-to-Use Format: No mixing or curing is required. Simply apply the mountant to the sample, add a coverslip, and begin imaging.
3. Glycerol-Based Formula: Maintains a refractive index suitable for fluorescence microscopy.
4. Signal Stability: Mounted samples remain stable for weeks, preserving signal intensity with minimal quenching.
5. Compatible Dyes: Designed to work efficiently with Alexa Fluor™ dyes and other fluorescent dyes.
6. Convenience: Comes in both standard and DAPI-containing formats for versatility in imaging applications.

Applications:

1. Fluorescence Microscopy: Ideal for studying fluorescently labeled cell cultures or tissue sections.
2. Alexa Fluor™ Dye Imaging: Optimized for use with Alexa Fluor and other common dyes.
3. Immunofluorescence: Perfect for experiments involving labeled antibodies and cellular markers.
4. Prolonged Imaging Studies: Suitable for imaging requiring multiple sessions, maintaining fluorescence stability over time.
5. Microscopy with Coverslips: Enables quick preparation of samples for immediate viewing under a microscope.

Limitations:

• Fluorescent Proteins: Not recommended for use with samples containing fluorescent proteins like GFP. SlowFade™ Diamond Antifade Mountant is suggested for these applications.
• Thicker Samples: For high-resolution imaging of thick tissue sections or 3D cultures with a focal depth of 0–500 μm, consider SlowFade™ Glass Antifade Mountant.

Product Specifications:

Instructions for Use:

1. Apply Mountant: Add a small drop of SlowFade Gold Antifade Mountant directly to the prepared sample on the slide.
2. Add Coverslip: Place a coverslip over the sample to spread the mountant evenly.
3. Image Immediately: Begin imaging under a fluorescence microscope.
4. Storage of Mounted Slides: Store slides at 2–8°C or at room temperature, protected from light. Mounted samples are stable for weeks.

Recommended Use Cases:

1. Basic Fluorescence Microscopy: Immediate imaging of cell or tissue samples post-staining.
2. Alexa Fluor Dye Studies: Long-term fluorescence stability for high-quality results in multicolor imaging.
3. Immunofluorescence Experiments: Mounting after antibody labeling protocols.
4. Cytological or Histological Analysis: Preservation of fluorescence signal in stained cells or tissues.
5. Educational and Research Labs: Ease of use makes it suitable for academic and professional research environments.

Storage Recommendations:

• Store at room temperature or frozen (-5°C to -30°C).
• Protect from light to prevent degradation of antifade properties.

For long-term studies or experiments involving fluorescent proteins or thick tissue samples, explore other SlowFade™ Antifade Mountants like SlowFade Diamond or SlowFade Glass for enhanced compatibility.