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Thermo Scientific™ Invitrogen™ Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488
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Excitation: 493 nm
Emission Max: 518 nm
Concentration: 2 mg/mL
Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-performance, highly cross-adsorbed fluorescent secondary antibody with enhanced brightness and minimal background for multiplex imaging
The Invitrogen™ Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 is a premium-grade fluorescent antibody designed for high-sensitivity detection of mouse primary antibodies in various immunoassays. Conjugated to Alexa Fluor™ Plus 488, this reagent delivers exceptionally bright, photostable green fluorescence with low background staining—ideal for demanding applications such as multiplex immunofluorescence and confocal microscopy.
This antibody is highly cross-adsorbed to eliminate cross-reactivity with bovine, goat, rabbit, rat, and human immunoglobulins, significantly improving specificity in complex tissue samples or co-staining protocols. It is suitable for immunocytochemistry (ICC/IF), immunohistochemistry (IHC), and Western blotting (WB) applications.
Key Features
- Superior Brightness: Alexa Fluor™ Plus 488 delivers enhanced signal intensity over conventional Alexa Fluor 488, ideal for detecting low-abundance targets.
- Highly Cross-Adsorbed: Cross-adsorbed against bovine, goat, rabbit, rat, and human IgG and serum to minimize background and cross-reactivity.
- Multiplex Compatibility: Ideal for multicolor fluorescence imaging and co-localization studies.
- Versatile: Validated for ICC/IF, IHC (paraffin and frozen), and Western blot applications.
- Trusted Quality: Affinity-purified polyclonal goat IgG, validated in 80+ figures and widely cited.
Applications & Recommended Dilutions
| Application | Suggested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| Western Blot (WB) | 0.1–0.4 µg/mL |
Dilution should be optimized based on experimental setup and imaging platform.
Technical Specifications
| Specification | Details |
|---|---|
| Catalog Numbers | A32723 (1 mg), A32723TR (100 µg) |
| Host Species | Goat |
| Target Species | Mouse |
| Antibody Type | Polyclonal, Whole Antibody |
| Target | IgG (Heavy and Light chains) |
| Conjugate | Alexa Fluor™ Plus 488 |
| Excitation/Emission Max | 493/518 nm |
| Concentration | 2 mg/mL |
| Form | Liquid |
| Purification | Affinity chromatography |
| Cross-Adsorbed Against | Bovine, Goat, Rabbit, Rat, Human IgG and serum |
| Storage Buffer | Proprietary buffer, pH 6.5 with preservatives |
| Contains | 0.016% Methylisothiazolone, 0.016% Bromonitrodioxane |
| Storage Conditions | 4°C, protect from light |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| RRID | AB_2633275 |
| Regulatory Status | Research Use Only (RUO) |
Product Benefits
- Enhanced signal clarity in high-background tissues
- Low cross-reactivity in multicolor staining workflows
- High specificity for detecting mouse primary antibodies
- Photostable signal ideal for long exposure and confocal imaging
- Reliable performance in both fixed cells and tissue sections
The Invitrogen™ Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 is the reagent of choice for researchers requiring maximum signal clarity and specificity in complex immunofluorescence applications. Its advanced formulation minimizes non-specific staining, enhances brightness, and ensures robust, reproducible results in even the most challenging experimental setups.
- Pack Size: 1mg 100 µg