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Thermo Scientific™ Hypersil GOLD™ PEI HILIC HPLC Columns
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Specifications:
| Application | HPLC |
| Storage Temperature | Ambient |
| Product Type | columns |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Analytical grade |
High-Performance Polyethyleneimine HILIC Columns for Highly Polar Analytes
The Thermo Scientific™ Hypersil GOLD™ PEI HILIC Columns are purpose-engineered for the retention and separation of highly polar and hydrophilic analytes that are often poorly retained or unresolved on traditional reversed-phase (C18) columns. The unique polyethyleneimine (PEI) stationary phase provides strong, predictable hydrophilic interaction chromatography (HILIC) selectivity, enabling the efficient analysis of analytes such as polar metabolites, sugars, nucleosides, small organic acids, and water-soluble drugs.
Offering particle sizes of 1.9 µm, 3 µm, and 5 µm, these columns support high-speed UHPLC separations, routine HPLC workflows, and LC-MS methodologies. Hypersil GOLD PEI HILIC columns deliver excellent peak shape, improved sensitivity, and high reproducibility, all without requiring derivatization or ion-pairing reagents—making them ideal for modern analytical laboratories.
Key Features & Benefits
1. Designed for Highly Polar Analytes
- Ideal for compounds not retained on C18
-
Excellent retention for:
✓ small polar pharmaceuticals
✓ polar metabolites
✓ sugars and amino sugars
✓ environmental polar contaminants
✓ hydrophilic drug discovery targets
2. Outstanding Peak Shape & MS Sensitivity
- PEI chemistry ensures stable hydrophilic interactions
- Produces sharp, narrow peaks for LC-MS detection
- No need for ion-pair reagents or derivatization
3. Broad Format Options
- Particle sizes: 1.9 µm (UHPLC), 3 µm, 5 µm
- Lengths: 10–250 mm
- IDs: 1.0–4.6 mm
Supports method development, high-throughput screening, and regulatory workflows.
4. Part of the Hypersil GOLD Family
- Exceptional reproducibility column-to-column
- Robust ultrahigh-purity silica
- Consistent selectivity and efficiency across batches
Typical Applications
- Pharmaceutical drug discovery (highly polar candidates)
- Metabolomics & polar metabolites
- Food safety testing (sugars, additives, polar compounds)
- Environmental analysis (polar pollutants, ionic species)
- LC-MS workflows requiring strong HILIC retention
General Specifications
| Parameter | Value |
|---|---|
| Column Type | HILIC |
| Stationary Phase | Polyethyleneimine (PEI) |
| Particle Sizes | 1.9, 3, 5 µm |
| Endcapped | No |
| Pore Size | 175 Å |
| Surface Area | 220 m²/g |
| Max Pressure | 400 bar (5800 psi) |
| pH Range | 2–8 |
| Temperature | 60°C |
| Packing Material | Fully Porous Ultrapure Silica |
| Carbon Load | 6% |
| Format | Analytical Columns |
| Product Line | Hypersil GOLD |
| Unit Size | Each |
1. Particle Size: 5 µm
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 26505-254630 | 250 | 4.6 | 5 µm |
| 26505-252130 | 250 | 2.1 | 5 µm |
| 26505-154630 | 150 | 4.6 | 5 µm |
| 26505-152130 | 150 | 2.1 | 5 µm |
| 26505-103030 | 100 | 3.0 | 5 µm |
| 26505-102130 | 100 | 2.1 | 5 µm |
| 26505-054630 | 50 | 4.6 | 5 µm |
| 26505-052130 | 50 | 2.1 | 5 µm |
| 26505-014001 | 10 | 4.0 | 5 µm |
2. Particle Size: 3 µm
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 26503-154630 | 150 | 4.6 | 3 µm |
| 26503-153030 | 150 | 3.0 | 3 µm |
| 26503-152130 | 150 | 2.1 | 3 µm |
| 26503-151030 | 150 | 1.0 | 3 µm |
| 26503-103030 | 100 | 3.0 | 3 µm |
| 26503-102130 | 100 | 2.1 | 3 µm |
| 26503-052130 | 50 | 2.1 | 3 µm |
| 26503-012101 | 10 | 2.1 | 3 µm |
| 26503-011001 | 10 | 1.0 | 3 µm |
3. Particle Size: 1.9 µm (UHPLC)
| Catalog Number | Length (mm) | ID (mm) | Particle Size |
|---|---|---|---|
| 26502-152130 | 150 | 2.1 | 1.9 µm |
| 26502-102130 | 100 | 2.1 | 1.9 µm |
| 26502-052130 | 50 | 2.1 | 1.9 µm |
Hypersil GOLD™ PEI HILIC columns provide strong, reliable retention of highly polar compounds that traditional C18 columns cannot retain. With excellent peak shape, MS-friendly chemistry, and multiple particle sizes, they are ideal for pharmaceutical development, metabolomics, food testing, and environmental analysis.
- Column Dimension: Particle Size: 5 μm; 250 x 2.1 mm Particle Size: 5 μm; 100 x 3.0 mm Particle Size: 3 μm; 150 x 1.0 mm Particle Size: 5 μm; 50 x 2.1 mm Particle Size: 5 μm; 50 x 4.6 mm Particle Size: 5 μm; 10 x 4.0 mm Particle Size: 5 μm; 100 x 2.1 mm Particle Size: 5 μm; 150 x 2.1 mm Particle Size: 5 μm; 150 x 4.6 mm Particle Size: 5 μm; 250 x 4.6 mm Particle Size: 3 μm; 50 x 2.1 mm Particle Size: 3 μm; 100 x 2.1 mm Particle Size: 3 μm; 100 x 3.0 mm Particle Size: 3 μm; 150 x 2.1 mm Particle Size: 3 μm; 150 x 3.0 mm Particle Size: 3 μm; 150 x 4.6 mm Particle Size: 3 μm; 10 x 2.1 mm Particle Size: 3 μm; 10 x 1.0 mm Particle Size: 1.9 μm; 50 x 2.1 mm Particle Size: 1.9 μm; 100 x 2.1 mm Particle Size: 1.9 μm; 150 x 2.1 mm