Thermo Scientific™ Glutaraldehyde, 25% aqueous solution

Glutaraldehyde is used for cross-linking proteins and polyhydroxy materials. Also used as a fixative for tissues and as an amine-reactive homobifunctional crosslinker. It is also used in coupling reaction of biotin hydrazides to nucleic acids which is useful for conjugating fluorescent hydrazides and hydroxylamines to DNA and for stabilization ofproteins on agarose beads,activation of polystyrene and glass, for immobilization of antibodies and antigens.

Applications

Glutaraldehyde is used for cross-linking proteins and polyhydroxy materials. Also used as a fixative for tissues, as an amine-reactive homobifunctional crosslinker, and as a disinfectant for medical equipment. It is also used in the coupling reaction of biotin hydrazides to nucleic acids, which is useful for conjugating fluorescent hydrazides and hydroxylamines to DNA and for stabilising proteins on agarose beads, activating polystyrene and glass, and for immobilising antibodies and antigens. It is a disinfectant, which is rapidly effective against vegetative forms of Gram-positive and Gram-negative bacteria.

Solubility

Miscible with water, ethanol, benzene, ether, acetone, dichloromethane, ethyl acetate, isopropanol, n-hexane and toluene.

Notes

Heat and air sensitive. Incompatible with strong acids, strong bases and strong oxidizing agents.

RUO – Research Use Only

General References:

1. For use in the Robinson synthesis of pseudopelletierine, see Acetone-1,3-dicarboxyl ic acid, A13742.
2. Reaction with (R)-(-)-2-Phenyl glycinol, A19030, gives an intermediate for asymmetric synthesis of alkaloids: Org. Synth. Coll., 9, 176 (1998).
3. Cho, K. L.; Hill, A. J.; Caruso, F.; Kentish, S. E. Chlorine Resistant Glutaraldehyde Crosslinked Polyelectrolyte Multilayer Membranes for Desalination. Adv. Mater. 2015, 27 (17), 2791-2796.
4. Vikram, A.; Bomberger, J. M.; Bibby, K. J. Efflux as a Glutaraldehyde Resistance Mechanism in Pseudomonas fluorescens and Pseudomonas aeruginosa Biofilms. Antimicrob. Agents Chemother. 2015, 59 (6), 3433-3440.