Thermo Scientific™ Gibco™ Gentamicin (50 mg/mL)

Gibco™ Gentamicin is a water-soluble antibiotic purified from the fungus Micromonospora purpurea. It acts by binding to the 30S ribosomal subunit of bacteria, inhibiting protein synthesis and leading to bacterial death. This antibiotic is effective against a wide range of gram-positive and gram-negative bacteria, making it an essential reagent for preventing bacterial contamination in cell culture workflows.

Key Features:

1. Broad-Spectrum Antibiotic:
   • Effective against both gram-positive and gram-negative bacteria.
2. Prevents Cell Culture Contamination:
   • Ensures contamination-free environments for cell growth and experimental reproducibility.
3. Flexible Working Concentrations:
   • Recommended concentrations range from 0.5 to 50 μg/mL, allowing use across various cell culture systems.
4. Sterile-Filtered Solution:
   • Ready-to-use, sterile-filtered formulation minimizes contamination risks.
5. Dual-Site Manufacturing:
   • cGMP compliant manufacturing facilities in the US and Scotland ensure product consistency and supply chain continuity.
6. Stable and Convenient:
   • Room temperature shipping and storage simplify handling and logistics.

Specifications:

Applications:

1. Contamination Prevention:
   • Prevent bacterial contamination in mammalian, insect, and other cell cultures.
2. Broad-Spectrum Antimicrobial Activity:
   • Suitable for use in diverse cell culture systems, targeting both gram-positive and gram-negative bacteria.
3. General Cell Culture Maintenance:
   • Essential for maintaining sterility in routine and large-scale cell culture workflows.
4. Research and Development:
   • Supports studies requiring contamination-free environments, such as drug screening and gene expression research.

Protocol Overview:

1. Prepare Culture Medium:
   • Dilute Gibco™ Gentamicin to the desired working concentration (0.5–50 μg/mL) in cell culture medium.
2. Sterilize if Necessary:
   • Ensure the medium remains sterile during preparation and handling.
3. Add to Cell Culture:
   • Incorporate the prepared medium into the cell culture environment.
4. Monitor Cultures:
   • Regularly assess cell cultures for contamination and optimal growth conditions.

Ordering Information:

Storage and Handling:

• Storage Conditions:
  • Store at 15°C to 30°C.
• Shelf Life:
  • 24 months from the date of manufacture.
• Shipping:
  • Ships at room temperature for convenience and stability.

Advantages:

1. Broad-Range Protection:
   • Prevents contamination by a wide variety of bacteria.
2. Convenience:
   • Ready-to-use liquid formulation simplifies experimental workflows.
3. Regulatory Compliance:
   • Manufactured in ISO 13485-certified, cGMP-compliant facilities.
4. Reliable Stability:
   • Long shelf life and stable at room temperature.
5. Flexible Use:
   • Effective in various cell culture formats, including flasks, plates, and suspension systems.

Related Products:

• Gibco™ Antibiotic-Antimycotic Solution: For broader antimicrobial protection.
• Gibco™ Penicillin-Streptomycin Solution: For dual bacterial coverage in cell culture.
• Gibco™ DMEM: High-quality culture medium for mammalian cells.

Regulatory Notes:

• For Research Use Only: Not for use in diagnostic or therapeutic applications.

The Gibco™ Gentamicin (50 mg/mL) offers a high-performance, broad-spectrum solution to maintain sterility in cell culture environments. Its stability, flexibility, and manufacturing quality make it a trusted choice for researchers worldwide.