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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Antifungals | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₁₃H₁₂F₂N₆O |
Fluconazole is a synthetic, broad-spectrum triazole antifungal compound supplied at ≥98% purity, widely used in microbiological research, drug discovery, and fungal susceptibility assays. It exerts its fungistatic activity primarily through inhibition of fungal cytochrome P-450 enzyme sterol 14α-demethylase, disrupting ergosterol biosynthesis—a vital component of fungal cell membranes.
Due to its selectivity, fluconazole exhibits significantly lower inhibition of mammalian cytochrome P450 enzymes, making it a reliable tool in antifungal assays and fungal pathogenesis research.
Key Features
- ≥98% HPLC purity
- Inhibits cytochrome P450 14α-demethylase in fungal cells
- Effective against Candida, Cryptococcus, and Coccidioides spp.
- Supplied as a white to off-white crystalline powder
- Highly soluble in DMSO, methanol, or ethanol for bioassays
- Ideal for use as positive control in antifungal screening experiments
- For Research Use Only (RUO)
Chemical & Physical Properties
| Property | Specification |
|---|---|
| CAS Number | 86386-73-4 |
| IUPAC Name | 2-(2,4-difluorophenyl)-1,3-bis(1H-1,2,4-triazol-1-yl)propan-2-ol |
| Molecular Formula | C₁₃H₁₂F₂N₆O |
| Molecular Weight | 306.27 g/mol |
| Purity (HPLC) | ≥97.5% |
| Melting Point | 138–142 °C |
| Appearance | White to off-white crystalline powder |
| Loss on Drying | ≤1% (105 °C, 3 h) |
| Solubility | Soluble in DMSO, methanol, ethanol |
| Storage | Ambient (store sealed and dry) |
Packaging Options
| Catalog No. | Quantity |
|---|---|
| 455480010 | 1 g |
| 455480050 | 5 g |
Applications
- Fungal susceptibility testing (e.g., MIC assays)
- Positive control in antifungal bioassays of plant or drug candidates
- Model antifungal agent for studying ergosterol pathway inhibition
- Used in research on Candida species, including C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis
- Studies on cytochrome P450 (CYP2C9) inhibition and drug metabolism
Mode of Action
Fluconazole inhibits fungal cytochrome P-450 sterol C-14 α-demethylation, a critical step in ergosterol synthesis. This leads to:
- Accumulation of toxic 14α-methyl sterols
- Disruption of cell membrane integrity
- Inhibition of fungal growth (fungistatic effect)
Storage & Handling
- Store in a cool, dry place in tightly closed container
- Use appropriate PPE when handling powders
- For laboratory research use only; not intended for therapeutic or diagnostic purposes
Supporting References
- Clancy, C.J. et al. Antimicrob. Agents Chemother. 2005, 49(8), 3171–3177.
- Matsui, K. et al. Mol. Pharmaceutics 2015, 12(7), 2418–2428.
- Bhardwaj, T. et al. J. Chem. Thermodyn. 2014, 78, 1–6.
Thermo Scientific’s Fluconazole, 98% offers a reliable and well-characterized antifungal agent for in vitro microbiological, biochemical, and pharmacological research. Its broad spectrum of activity, high purity, and defined mode of action make it a go-to control reagent for researchers investigating antifungal efficacy, resistance mechanisms, and drug-pathogen interactions.
- Pack Size: 1g 5g 25g