Thermo Scientific™ Crystal violet, pure indicator

Thermo Scientific™ Crystal Violet is a high-purity dye certified for use in classic histological and microbiological staining applications. It is widely recognized as the primary stain in Gram staining, playing a central role in differentiating Gram-positive from Gram-negative bacteria. Beyond microbiology, it is validated for use in Flemming’s triple stain with iodine, Lieb’s amyloid stain, and Newton’s crystal violet-iodine technique for chromatin and nucleoli.

Its versatility extends into cell viability studies, DNA visualization in agarose gels, and chromosomal staining in plants and microorganisms, making it an indispensable reagent across microbiology, cytology, and molecular biology.

Key Features

• Certified purity ensures reproducible and accurate Gram stain results.
• Versatile applications, including Gram staining, Flemming’s triple stain, amyloid and chromatin staining, and viability assays.
• Suitable for DNA visualization in agarose gels at high DNA concentrations.
• Widely used in research on cell migration, invasion, and colorimetric viability assays.
• Quality assured through compliance with infrared and UV spectral standards, with guaranteed sensitivity as an indicator.
• Packaged in durable glass bottles with SDS and certificates of analysis traceable by lot number.

Technical Specifications

Hazard Classification

• Irritant
• Health hazard
• Corrosive
• Environmental hazard

Available Pack Sizes

Applications

• Microbiology: Primary Gram stain for bacterial differentiation.
• Histology: Used in Flemming’s triple stain, amyloid staining, chromatin, and nucleoli visualization.
• Cell biology: Applied in cell viability assays, migration, and invasion studies.
• Molecular biology: Suitable for DNA visualization in agarose gels, particularly at high DNA concentrations.
• Plant cytology: Effective for staining plant chromosomes.

Thermo Scientific™ Crystal Violet, Pure Indicator is a trusted, high-quality dye designed for precision, reproducibility, and versatility in laboratory applications. From Gram staining to advanced cytological and molecular biology workflows, it consistently delivers reliable results. Supported with certified compliance, safety documentation, and full traceability, it is the preferred choice for laboratories requiring dependable and professional staining solutions.