Thermo Scientific™ Buffer, HEPES solution pH 7.3 (1 mol/L), Molecular Biology Grade, Ultrapure

Thermo Scientific's HEPES buffer solution is a high-quality, ready-to-use solution designed for a wide range of biological and molecular biology applications. Its ultrapure formulation ensures precision and reproducibility in sensitive experiments requiring a stable pH environment.

Key Features

1. Ready-to-Use Solution:
   • Pre-prepared 1 mol/L solution for convenience and consistency.
   • Reduces preparation time and errors associated with manual buffer preparation.
2. Precise pH Control:
   • pH adjusted to 7.3, ideal for many biological and biochemical reactions.
   • Stable pH under experimental conditions, including non-CO₂ incubator environments.
3. Ultrapure Quality:
   • Molecular biology grade ensures minimal contamination, suitable for sensitive applications.
   • Free from DNase, RNase, and protease contamination.
4. Wide Application Range:
   • Suitable for cell culture, protein and nucleic acid research, and enzymatic assays.

Chemical and Physical Properties

Applications

1. Cell Culture:
   • Maintains pH stability during experiments outside CO₂ incubators.
   • Supports mammalian and microbial cell growth in culture systems.
2. Biochemical Assays:
   • Provides consistent pH for enzymatic reactions.
   • Ideal for protein activity studies and nucleic acid manipulations.
3. Molecular Biology:
   • Compatible with DNA, RNA, and protein research.
   • Suitable for electrophoresis and other analytical techniques.
4. Diagnostic Research:
   • Used in assay development for accurate and reproducible results.

Advantages

• Convenience: Pre-made solution eliminates the need for weighing and dissolving HEPES powder, ensuring uniformity and reliability.
• pH Stability: Ensures accurate buffering even under fluctuating environmental conditions.
• High Purity: Molecular biology grade ensures the absence of contaminants that may interfere with experiments.
• Versatility: Compatible with a wide range of biological and molecular biology applications.

Preparation and Usage

• Concentration: 1 mol/L solution, ready to use without dilution.
• Usage Recommendations:
  • Use directly in cell culture media, reaction mixtures, or assay buffers requiring a pH of 7.3.
  • Mix gently before use to ensure homogeneity.

Storage and Stability

• Storage Temperature: Room temperature (15–25 °C).
• Shelf Life: Stable for an extended period under recommended storage conditions.
• Packaging: Supplied in leak-proof, laboratory-grade containers for easy handling.

Safety Information

• Hazard Classification: Not hazardous under standard handling conditions.
• Precautions:
  • Wear gloves and goggles when handling to avoid accidental spills.
  • Use in a well-ventilated workspace or under a fume hood if necessary.

The Thermo Scientific HEPES Buffer Solution (1 mol/L, pH 7.3, Molecular Biology Grade, Ultrapure) is a reliable and high-quality buffer ideal for precision-focused research in molecular biology and biochemistry. Its ultrapure formulation, ease of use, and stable pH make it a versatile solution for experiments requiring accurate and consistent pH control.