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Specifications:
| Application | Western Blot, ELISA-Based Assays | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Blocker™ Casein is a ready-to-use 1% (w/v) purified casein solution designed for blocking steps in Western blotting, ELISA, immunohistochemistry (IHC), and nucleic acid detection assays.
Unlike serum- or milk-based blocking buffers, this single-protein formulation minimizes cross-reactivity, ensuring cleaner backgrounds and higher assay specificity.
Key Features
- Purified casein blocking buffer – reduces nonspecific binding compared to serum or milk solutions.
- Ready-to-use format (1X, 1% casein) – saves time, no additional preparation required.
- Versatile – suitable for Western blot, ELISA, IHC, and nucleic acid detection assays.
- Stable formulation – thimerosal-free and safe to use in sensitive immunoassays.
- Flexible – can be diluted further as needed for specific applications.
Specifications
| Property | Detail |
|---|---|
| Concentration | 1X (1% casein solution) |
| Form | Liquid |
| Buffers Available | PBS or TBS |
| Storage | Store at 4°C |
| Applications | Western Blot, ELISA, IHC, nucleic acid assays |
| Product Line | Blocker™ |
| Safety | Thimerosal-free, safe formulation |
| For Use | Research Use Only |
Catalog Numbers & Formats:
| Catalog Number | Quantity | Buffer |
|---|---|---|
| 37528 | 1 L | PBS |
| 37528X3 | 3 × 1 L | PBS |
| 37582 | 100 mL | PBS |
| 37532 | 1 L | TBS |
| 37583 | 100 mL | TBS |
Applications
- Blocking nonspecific binding in Western blotting.
- Use as a blocking reagent in ELISA workflows.
- Suitable for IHC tissue staining to reduce background.
- Preventing nonspecific probe binding in nucleic acid detection assays.
The Thermo Scientific™ Blocker™ Casein provides a convenient, high-performance blocking solution for immunoassays and nucleic acid detection techniques. With its ready-to-use 1% purified casein formulation in PBS or TBS, it delivers lower cross-reactivity, cleaner backgrounds, and more reproducible assay results than traditional milk or serum-based blocking buffers.
- Buffer Type: 100 mL in PBS Buffer 100 mL in TBS Buffer 1L in PBS Buffer 1L in TBS Buffer 3 x 1L in PBS Buffer