Applied Biosystems™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Enhanced chemiluminescent detection for nitrocellulose and PVDF membranes

The Applied Biosystems™ Nitro-Block-II™ Enhancer is a concentrated luminescence enhancer designed to significantly increase the alkaline phosphatase signal when used with CSPD™ or CDP-Star™ chemiluminescent substrates in membrane blotting assays.

Specially formulated for protein and nucleic acid blotting, this enhancer optimizes chemiluminescent signal intensity, reducing exposure times and improving detection sensitivity. It is suitable for both nitrocellulose and PVDF membranes, making it a valuable tool for western blot and other membrane-based detection workflows.

Key Features & Benefits

• Signal Amplification – Increases chemiluminescent signal intensity for improved detection sensitivity.
• Optimized Performance – Designed for dioxetane-based substrates (CSPD™ and CDP-Star™).
• Faster Detection – Reduces exposure times from hours to minutes.
• Membrane Versatility – Compatible with both nitrocellulose and PVDF membranes.
• Convenient Format – Concentrated liquid formulation for easy handling.

Performance Advantages

• Nitrocellulose membranes: Shortens exposure times from potentially hours to 10–45 minutes when used with enhancer.
• PVDF membranes: Up to 10× faster detection with exposure times as short as 15 seconds to 15 minutes.
• Substrate compatibility:
  • CSPD™ substrate – Use with both nitrocellulose and PVDF membranes.
  • CDP-Star™ substrate – Use only with nitrocellulose membranes.

Applications

• Western blotting (protein detection)
• Nucleic acid membrane blotting assays
• High-sensitivity chemiluminescent detection workflows
• Reduction of imaging times in AP-based blotting assays

Technical Specifications

The Applied Biosystems™ Nitro-Block-II™ Enhancer delivers exceptional improvements in chemiluminescent detection sensitivity for alkaline phosphatase-based membrane blotting assays. Whether used on nitrocellulose or PVDF membranes, it enables faster imaging times, clearer results, and more efficient workflows. Ideal for western blot and nucleic acid blotting applications, this enhancer is a powerful addition to any molecular biology laboratory seeking reliable and high-performance detection results.