Human metapneumonia virus igM, HMPV Elisa kit

The Human Metapneumovirus IgM (HMPV-IgM) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of HMPV-IgM antibodies in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into HMPV infections, acute immune responses, and epidemiological studies.

Purpose

• Quantification of HMPV-IgM Levels:
  • Detect and measure HMPV-IgM concentrations in human biological samples to study acute immune responses and HMPV infection prevalence.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a calibrated ELISA technique for accurate and reliable quantification of HMPV-IgM. The process includes:

1. Calibration Standards:
   • A set of HMPV-IgM calibration standards is included to generate a standard curve.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells. HMPV-IgM in the sample binds to the specific antigen pre-coated on the wells.
3. Color Development:
   • Following processing, the reaction changes color from blue to yellow after adding the stop solution.
4. Optical Density Measurement:
   • Optical density (OD) is measured at 450 nm using a spectrophotometer.
5. Concentration Determination:
   • The concentration of HMPV-IgM in the samples is calculated by comparing the OD values to the standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of HMPV-IgM with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of HMPV-IgM concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa plate.
• HMPV-IgM calibration standards for standard curve generation.
• HRP-conjugated detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Instruction manual.

Applications

• HMPV Infection Research:
  • Study acute immune responses to human metapneumovirus (HMPV) infections.
• Serological Studies:
  • Monitor HMPV-IgM prevalence and acute-phase immune response in populations.
• Epidemiological Research:
  • Investigate outbreaks and geographic spread of HMPV infections.
• Clinical Diagnostics Research:
  • Support research into acute HMPV infections and related immune responses.
• Vaccine Development:
  • Evaluate immune responses to potential HMPV vaccines.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of HMPV-IgM antibodies.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate and wash as directed.
3. Add TMB substrate to develop color.
4. Stop the reaction and measure OD at 450 nm.
5. Calculate HMPV-IgM concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Metapneumovirus IgM (HMPV-IgM) ELISA Kit is a vital tool for researchers studying HMPV infections, acute immune responses, and vaccine efficacy. Its precision, user-friendly design, and broad compatibility make it indispensable for clinical and experimental applications.