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Invitrogen™ iBind™ Fluorescent Detection (FD) Solution Kit
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Specifications:
| Application | Western Blot | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Optimized fluorescent detection reagents for the iBind™ Western Device
The Invitrogen™ iBind™ Fluorescent Detection (FD) Solution Kit is formulated for use with the iBind™ Western Device and is specifically optimized for western blotting with fluorescent-conjugated antibodies. It supports a wide range of fluorescence-based applications, including infrared detection and dual-wavelength semi-quantitative analysis.
The FD Solution Kit provides the ideal viscosity for blocking, washing, and antibody dilution, ensuring optimal reagent flow and performance during the automated sequential lateral flow (SLF) process. When loaded into the iBind™ Western Device, the solutions are evenly delivered to the membrane through the iBind™ Card’s glass fiber matrix, improving antigen–antibody interactions and enhancing detection sensitivity.
Each kit contains enough reagents to process 10 mini-membrane reactions. It is intended for research use only and must be used with both the iBind™ Western Device and iBind™ Cards.
Key Features & Benefits
- Fluorescence-Optimized – Designed for fluorescent-conjugated antibodies, including infrared detection workflows.
- Dual-Wavelength Ready – Suitable for semi-quantitative dual-wavelength analysis.
- Consistent Reagent Delivery – Ideal viscosity ensures optimal performance in SLF-based automation.
- Reproducible Results – Minimizes variability and improves sensitivity for fluorescence-based detection.
- Complete Kit – Contains blocking, washing, and antibody dilution solutions.
- Membrane Compatibility – Works with both nitrocellulose and PVDF membranes.
Applications
- Fluorescent western blot detection workflows
- Infrared-based protein detection
- Dual-wavelength semi-quantitative protein analysis
- Automated antibody incubation and wash steps
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Fluorescent Detection Kit |
| Product Line | iBind™ |
| For Use With (Application) | Western blot |
| For Use With (Equipment) | iBind™ Western Device |
| Kit Contents | 1 bottle 5X buffer, 2 vials 100X additive, 1 vial 10% SDS |
| Sufficient For | 10 mini-membrane reactions |
| Detection Method | Fluorescence |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Unit Size | 1 kit |
| Quantity | 1 kit |
| Catalog Number | SLF1019 |
| Storage Conditions | Store at 2–8°C |
The Invitrogen™ iBind™ Fluorescent Detection (FD) Solution Kit delivers optimized reagents for high-quality, reproducible fluorescence-based western blot detection. With tailored formulations for fluorescent-conjugated antibodies and compatibility with both nitrocellulose and PVDF membranes, it ensures consistent results when paired with the iBind™ Western Device and iBind™ Cards, making it an essential component for advanced fluorescent immunodetection workflows.