Human Thyroxine antibody (TAb) ELISA Kit

The Human Thyroxine Antibody (TAb) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of thyroxine antibody (TAb) levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into autoimmune thyroid disorders and thyroid function.

Purpose

• Quantification of TAb Levels:
  • Detect and measure thyroxine antibody concentrations in human biological samples for research into autoimmune thyroid diseases and immune responses.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to ensure accurate and reliable quantification of TAb. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antigen specific to TAb.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where TAb in the sample binds to the immobilized antigen.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antigen specific to TAb is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing TAb.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • TAb concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of TAb with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of TAb concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• TAb standards for standard curve generation.
• HRP-conjugated TAb detection antigen.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Autoimmune Thyroid Disease Research:
  • Study TAb as a biomarker for autoimmune thyroid disorders such as Hashimoto’s thyroiditis and Graves’ disease.
• Endocrine Studies:
  • Investigate TAb’s role in thyroid hormone regulation and immune responses.
• Clinical Diagnostics Research:
  • Assess TAb as a marker for thyroid autoimmunity and related conditions.
• Therapeutic Development:
  • Evaluate treatments targeting autoimmune thyroid disorders and immune modulation.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of TAb.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated TAb antigen.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate TAb concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Thyroxine Antibody (TAb) ELISA Kit is a vital tool for researchers investigating autoimmune thyroid disorders, immune responses, and thyroid health. Its precision, user-friendly design, and broad compatibility make it indispensable for clinical and experimental applications.