Human Cardiac Troponin T (Ctnt) Elisa Kit, 96 Tests

Human Cardiac Troponin T (cTnT) ELISA Kit

The Human Cardiac Troponin T (cTnT) ELISA Kit is a highly sensitive and reliable tool designed for the quantitative detection of cTnT levels in human serum, plasma, urine, cell culture supernatants, and tissue samples. This kit uses the Sandwich-ELISA technique and is ideal for research in cardiac health, myocardial injury, and related conditions.

Purpose

• Quantification of cTnT Levels:
  • Measures cTnT concentrations as a key biomarker for cardiac muscle damage.
• Sample Compatibility:
  • Valid for human serum, plasma, urine, cell supernatants, and tissue samples.

Principle

The assay employs a Sandwich-ELISA technique to detect and quantify cTnT with high specificity and sensitivity. Below is the detailed process:

1. Pre-Coated Plate:
   • Microtiter plate wells are pre-coated with an antibody specific to cTnT.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing cTnT in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to cTnT is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in positive wells.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. The optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • cTnT concentrations are calculated by comparing OD values to a standard curve.

Sample Preparation

1. Serum Preparation:

• Allow blood to clot at room temperature for 10–20 minutes.
• Centrifuge at 2,000–3,000 rpm for 20 minutes to separate serum.
• Re-centrifuge if precipitates form during storage.

2. Plasma Preparation:

• Collect blood in anticoagulant tubes (e.g., EDTA, citrate).
• Incubate at room temperature for 10–20 minutes.
• Centrifuge at 2,000–3,000 rpm for 20 minutes to obtain plasma.
• Re-centrifuge if necessary.

3. Urine Samples:

• Collect urine in aseptic tubes.
• Centrifuge at 2,000–3,000 rpm for 20 minutes and carefully collect the supernatant.
• Re-centrifuge if precipitates form during storage.

4. Cell Samples:

• For cell secretions, centrifuge culture supernatants at 2,000–3,000 rpm for 20 minutes.
• For intracellular components, dilute cells in PBS (pH 7.2–7.4) and disrupt using freeze-thaw cycles. Centrifuge as above.

5. Tissue Samples:

• Cut, weigh, and freeze tissues in liquid nitrogen; store at -80°C.
• Homogenize tissue in PBS (pH 7.4) at 4°C.
• Centrifuge at 2,000–3,000 rpm for 20 minutes to collect the supernatant.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of cTnT with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, urine, cell supernatants, and tissue homogenates.
3. Quantitative Results:
   • Provides precise measurement of cTnT concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• cTnT standards for standard curve generation.
• HRP-conjugated cTnT detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Cardiac Health Research:
  • Investigate cardiac muscle damage and related disorders.
• Myocardial Infarction Studies:
  • Monitor Troponin T as a biomarker for heart attack.
• Clinical Diagnostics Research:
  • Assess cTnT levels in various cardiac conditions.
• Drug Development:
  • Evaluate the impact of cardiotoxic drugs on heart health.
• Cardiac Disease Monitoring:
  • Study the progression of heart failure and related diseases.

Advantages

1. Accurate and Reliable:
   • High specificity for cTnT ensures precise quantification.
2. Convenient:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Reproducible:
   • Consistent performance across multiple experiments.
4. Versatile:
   • Compatible with various biological sample types.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles.

The Human Cardiac Troponin T (cTnT) ELISA Kit is a reliable, sensitive, and specific tool for measuring cTnT in biological samples. Its user-friendly design, wide compatibility, and precise quantification make it an invaluable resource for research into cardiac health, myocardial injury, and related disorders.