Human Thyroxine - T4 Elisa Kit, 96 Tests In 96 Well Plate

The Human Thyroxine (T4) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of thyroxine (T4) levels in human serum, plasma, urine, culture media, and other biological fluids. This kit is essential for research into thyroid function, hormonal balance, and related endocrine disorders.

Purpose

• Quantification of T4 Levels:
  • Detect and measure T4 concentrations in human biological samples for research into thyroid hormone regulation and metabolic processes.
• Sample Compatibility:
  • Suitable for serum, plasma, urine, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the accurate and reliable quantification of T4. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to T4.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where T4 in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to T4 is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing T4.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • T4 concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of T4 with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of T4 concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• T4 standards for standard curve generation.
• HRP-conjugated T4 detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Thyroid Function Research:
  • Study T4’s role in thyroid hormone regulation and metabolism.
• Endocrine Studies:
  • Investigate T4 levels in hypothyroidism, hyperthyroidism, and subclinical thyroid dysfunctions.
• Clinical Diagnostics Research:
  • Assess T4 as a biomarker for thyroid disorders and hormone imbalances.
• Metabolic Research:
  • Explore T4’s impact on metabolic rate and energy balance.
• Therapeutic Development:
  • Evaluate treatments targeting thyroid hormone production and regulation.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of T4.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated T4 antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate T4 concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Thyroxine (T4) ELISA Kit is a vital tool for researchers studying thyroid function, hormone regulation, and endocrine disorders. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.