Human Neonatal Thyroxine, NN-T4 ELISA Kit

The Human Neonatal Thyroxine (NN-T4) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of neonatal thyroxine (T4) levels in human serum, plasma, urine, culture media, and other biological fluids. This kit is essential for research into neonatal thyroid function, developmental health, and related disorders.

Purpose

• Quantification of Neonatal Thyroxine Levels:
  • Detect and measure NN-T4 concentrations in human biological samples for research into neonatal thyroid function and hormone regulation.
• Sample Compatibility:
  • Suitable for serum, plasma, urine, culture media, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique to ensure accurate and reliable quantification of NN-T4. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to NN-T4.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where NN-T4 in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to NN-T4 is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing NN-T4.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • NN-T4 concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of NN-T4 with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of NN-T4 concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• NN-T4 standards for standard curve generation.
• HRP-conjugated NN-T4 detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Neonatal Thyroid Function Research:
  • Study NN-T4’s role in neonatal thyroid hormone regulation and metabolism.
• Developmental Health Studies:
  • Investigate NN-T4 levels in neonatal growth and developmental processes.
• Clinical Diagnostics Research:
  • Assess NN-T4 as a biomarker for neonatal thyroid disorders and hormone imbalances.
• Reproductive and Prenatal Health Research:
  • Explore the relationship between maternal and neonatal thyroid function.
• Therapeutic Development:
  • Evaluate treatments targeting neonatal thyroid hormone production and regulation.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of NN-T4.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated NN-T4 antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate NN-T4 concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Neonatal Thyroxine (NN-T4) ELISA Kit is a vital tool for researchers studying neonatal thyroid health, hormone regulation, and developmental disorders. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.