Human Hepatitis C Virus Igg, Hcv-Igg Elisa Kit, 96 Tests

The Human Hepatitis C Virus IgG (HCV-IgG) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of HCV-IgG antibodies in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research in hepatitis C virus (HCV) infections, immune response, and epidemiological studies.

Purpose

• Quantification of HCV-IgG Levels:
  • Detects and measures HCV-IgG antibody concentrations to monitor immune responses to HCV infection.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the specific and accurate measurement of HCV-IgG. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antigen specific to HCV-IgG.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where HCV-IgG antibodies in the sample bind to the immobilized antigen.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antigen specific for HCV-IgG is added, forming a sandwich complex with the captured antibodies.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with HCV-IgG.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • HCV-IgG concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of HCV-IgG with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of HCV-IgG concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• HCV-IgG standards for standard curve generation.
• HRP-conjugated HCV antigen detection reagent.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Hepatitis C Virus Research:
  • Study immune responses to HCV infections.
• Serological Studies:
  • Assess past or current HCV infections through IgG detection.
• Epidemiological Studies:
  • Investigate HCV prevalence in populations.
• Clinical Diagnostics Research:
  • Support diagnostics for HCV-related conditions.
• Vaccine Development:
  • Evaluate immune responses to potential HCV vaccines.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of HCV-IgG.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated HCV antigen.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate HCV-IgG concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Hepatitis C Virus IgG (HCV-IgG) ELISA Kit is a valuable tool for the detection and quantification of HCV-IgG in biological samples. Its high sensitivity, specificity, and user-friendly design make it indispensable for research in HCV infections, immune response, and epidemiology.