Human C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Human C-Telopeptide of Type I Collagen (CTX-I) ELISA Kit

The Human C-Telopeptide of Type I Collagen (CTX-I) ELISA Kit is a reliable and sensitive assay designed for the quantitative measurement of CTX-I levels in human serum, plasma, culture media, and other biological fluids. Using a Sandwich-ELISA technique, this kit provides precise and reproducible results, making it ideal for research in bone metabolism, osteoporosis, and collagen turnover.

Purpose

• Quantification of CTX-I Levels:
  • Measures CTX-I concentrations as a biomarker for bone resorption and collagen degradation.
• Sample Compatibility:
  • Designed for serum, plasma, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to provide specific and accurate quantification of CTX-I. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to CTX-I.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing CTX-I in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific for CTX-I is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to reduce background noise.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with CTX-I.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • CTX-I concentrations are determined by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of CTX-I with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of CTX-I concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• CTX-I standards for standard curve generation.
• HRP-conjugated CTX-I detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Bone Metabolism Research:
  • Study bone resorption markers in metabolic bone diseases.
• Osteoporosis Studies:
  • Monitor collagen degradation and assess treatment efficacy.
• Collagen Turnover Analysis:
  • Investigate collagen breakdown in various conditions, including arthritis and cancer metastasis.
• Drug Development:
  • Evaluate the impact of therapeutic interventions targeting bone resorption.
• Clinical Diagnostics:
  • Support diagnostics for bone disorders and metabolic diseases.

Advantages

1. High Accuracy:
   • Ensures precise and reproducible quantification of CTX-I.
2. Convenient:
   • Ready-to-use reagents reduce preparation time.
3. Versatile:
   • Compatible with a variety of biological sample types.
4. Reliable:
   • Provides consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated CTX-I antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate CTX-I concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human C-Telopeptide of Type I Collagen (CTX-I) ELISA Kit is an essential tool for quantifying CTX-I levels in biological samples. Its high sensitivity, specificity, and user-friendly design make it invaluable for research in bone metabolism, collagen turnover, and related disorders.