Sephadex® LH-20 Size Exclusion Resin, 18 - 111 µm Particle size

Sephadex™ LH-20 is a versatile chromatography medium designed for molecular sizing and separation of natural products such as steroids, terpenoids, lipids, hormones, vitamins, low molecular weight peptides, and other small biomolecules. It is prepared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium, giving it unique hydrophilic and lipophilic properties. These properties make it highly effective for separating molecules with close molecular weights, offering powerful chromatographic selectivity.

Key Features

1. Hydrophilic and Lipophilic Matrix:
   • Dual nature (hydrophilic and lipophilic) ensures enhanced selectivity and compatibility with both polar organic solvents and aqueous solvent mixtures.
2. High Chromatographic Resolution:
   • Optimized for molecular sizing of compounds up to 5 kDa (small organic molecules) and 4 kDa (peptides).
   • Ensures clear fractionation of closely related molecular species.
3. Wide Solvent Compatibility:
   • Suitable for polar organic solvents and aqueous mixtures, making it ideal for diverse separation applications, including non-polar environments.
4. Reproducibility and Performance:
   • Exceptional batch-to-batch reproducibility ensures consistent results for analytical and industrial-scale applications.
   • Flow rate optimized to <60 cm/hour for effective separations.
5. Durable Matrix:
   • Made from hydroxypropylated cross-linked dextran beads that yield a robust polysaccharide network.
   • Physico-chemical stability across a pH range of 2–13 and a temperature range of 4–30°C.
6. Particle and Pore Characteristics:
   • Particle size: 18–111 μm.
   • Pore size: 60 Å, optimized for small biomolecules and peptides.
   • Surface area of 480–540 m²/g ensures high capacity for separation.

Applications

Sephadex™ LH-20 is ideal for a wide range of applications in both research and industry, including:

1. Natural Product Purification:
   • Fractionation of natural compounds such as lipids, terpenoids, and steroids.
   • Effective in separating complex mixtures of biomolecules.
2. Biopharmaceutical Research:
   • Purification of low molecular weight peptides, small organic molecules, and hormones.
   • Useful as a polishing step in the preparation of diastereomers.
3. Analytical and Industrial-Scale Chromatography:
   • Ideal for both analytical and preparative separations.
   • Frequently used in the preparation of pharmaceutical intermediates.
4. Versatile Laboratory Use:
   • Supports both initial purification steps and final polishing in chromatography workflows.
   • High selectivity ensures excellent results for molecular fractionation.

Specifications

Available Configurations

1. Sephadex™ LH-20, 25 g
   • Catalog Number: 17-0090-10
2. Sephadex™ LH-20, 100 g
   • Catalog Number: 17-0090-01
3. Sephadex™ LH-20, 500 g
   • Catalog Number: 17-0090-02

Usage Guidelines

1. Equilibrate the column with a compatible solvent or solvent mixture before use.
2. Use a flow rate of less than 60 cm/hour for optimal separation results.
3. For maximum performance, avoid exposure to conditions outside the specified pH and temperature ranges.
4. Suitable for multiple purification steps, including fractionation and final polishing of samples.

Key Benefits

• Excellent separation efficiency due to high chromatographic selectivity.
• Versatile use across different solvents and molecular species.
• Durable matrix for reproducible results in every batch.
• Simplifies the purification process, reducing the need for multiple separation steps.

Industries

1. Pharmaceuticals: For purifying active pharmaceutical ingredients (APIs) and intermediates.
2. Biotechnology: For peptide and biomolecule purification.
3. Nutraceuticals: For isolating vitamins, lipids, and other compounds.
4. Academic Research: Widely used in natural product and biomolecule research.

Sephadex™ LH-20 is a trusted chromatography solution for precision molecular separation, delivering consistent and reliable results across a wide variety of applications.