Sansure Measles and Rubella virus (MeV/RV) PCR Detection kit

Comprehensive, Rapid, and Reliable Detection of MeV and RV RNA in Clinical Samples

The Sansure Biotech Measles and Rubella Virus (MeV/RV) RNA Diagnostic Kit is a molecular diagnostic solution designed for the qualitative detection of Measles virus (MeV) and Rubella virus (RV) RNA in human throat swab, serum, and urine samples. It uses a real-time polymerase chain reaction (qPCR) technique based on fluorescent probe technology. This kit incorporates Sansure’s proprietary One-Tube Fast Release Technology, which enables viral RNA extraction, reverse transcription, and amplification in a single closed tube, ensuring workflow efficiency, speed, and biosafety.

The kit is specifically optimized for clinical diagnostic laboratories, reference labs, and public health institutions involved in infectious disease surveillance and outbreak response.

Key Features and Benefits

✅ Dual-Pathogen Detection

Simultaneous detection of Measles virus and Rubella virus in a single reaction. This saves time, reagents, and effort, particularly in regions where both viruses circulate or during co-infection investigations.

✅ One-Tube Workflow

The kit integrates viral lysis, nucleic acid release, reverse transcription, and real-time PCR amplification into a single closed-tube reaction. This:

• Reduces contamination risk.
• Simplifies operation.
• Shortens hands-on time.

✅ High Sensitivity and Specificity

• Limit of Detection (LoD):
  • Measles Virus: 200 copies/mL
  • Rubella Virus: 500 copies/mL
• Designed with highly specific primers and probes targeting conserved regions of the MeV and RV genomes.

✅ Internal Control for Process Validation

An internal control (often targeting the human RNase P gene) is included in each reaction tube to:

• Monitor sample quality.
• Validate RNA extraction and amplification.
• Prevent false negatives due to sample degradation or PCR inhibition.

✅ Multiple Sample Compatibility

Validated for use with:

• Throat swabs
• Serum samples
• Urine samples

This flexibility supports early-stage, mid-stage, and convalescent-phase diagnosis.

✅ Fast Turnaround Time

• Total reaction time: Approximately 60 minutes
• Enables rapid clinical decision-making and timely public health intervention.

✅ Broad Platform Compatibility

Compatible with commonly used real-time PCR instruments, including:

• ABI 7500
• Bio-Rad CFX96
• Roche LightCycler 480
• Sansure SLAN-96P
• And other qPCR cyclers supporting FAM, ROX, and VIC/HEX channels.

Technical Specifications

Comparison with Related Kits

Unique Advantages

🔬 Integrated Workflow

The complete one-tube reaction not only simplifies operations but also minimizes operator variability, ensuring consistent and reproducible results across technicians and laboratories.

🧪 High Biosafety

The closed-tube format significantly reduces the risk of aerosol contamination, which is a common concern in open PCR systems, making this kit ideal for point-of-care and field applications.

📈 Public Health Utility

The kit supports mass screening during outbreaks or routine surveillance campaigns, particularly in immunization programs and elimination monitoring.

🌍 Versatile Application

Suited for:

• Clinical diagnosis of measles and rubella
• Investigation of fever and rash illnesses
• Outbreak response
• Congenital rubella syndrome (CRS) surveillance

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