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Virplex® MPOX Clades RealTime PCR Kit, 24 Rxn
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Targets
MPOX virus (F3L gene), MPOX Clade I (OPG027 gene), MPOX Clade II (OPG065 gene), Human RNAse P (internal control)
Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® MPOX Clades RealTime PCR Kit is a multiplex real-time PCR assay designed for the qualitative detection of the MPOX (monkeypox) virus and its classification into Clade I or Clade II using human skin lesion samples. It provides a complete molecular solution with lyophilized reagents, internal controls, and validated compatibility across a wide range of extraction systems and PCR thermocyclers.
Key Features
- Multiplex PCR – one single reaction tube per sample.
- Clade differentiation – simultaneous detection of MPOX Clade I and Clade II.
- Broad qPCR compatibility – suitable for FAM, Cy5, HEX (VIC), and Texas Red channels.
- Internal control (RNAse P) – monitors sample quality, nucleic acid integrity, and inhibition.
- Rapid results – turnaround time of 1 h 30 min.
- Lyophilized reagents – master mix, positive control, and negative control for long-term stability and reduced cold chain costs.
- Research Use Only (RUO) – for laboratory and research applications.
Applications
- Detection and differentiation of MPOX virus Clade I and Clade II in suspected clinical cases.
- Vesicular smear samples collected on transport medium or dry swabs.
- Outbreak investigation and epidemiological surveillance.
- Quality control and validation in molecular virology laboratories.
- Research use for monitoring virus evolution and lineage spread.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® MPOX Clades RealTime PCR Kit |
| Reference | RTPCR034-R |
| Nº Tests | 24 |
| Format | Multiplex real-time PCR assay |
| Class | RUO – Research Use Only |
| Sample Type | Human skin lesion swabs / vesicular smear |
| Targets | MPOX virus (F3L gene), MPOX Clade I (OPG027 gene), MPOX Clade II (OPG065 gene), Human RNAse P (internal control) |
| Result Interpretation | Texas Red/ROX (Monkeypox virus), HEX/VIC (Clade I), FAM (Clade II), Cy5 (RNAse P) |
| Time to Results | ~1 h 30 min |
| Reagents | Single mix, reconstitution solution, positive control, negative control |
| Viral Load (Control) | 10,000–20,000 copies/µL |
| Storage | Lyophilized reagents, stable at ambient conditions |
Compatibility
Extraction Systems:
- BioMérieux NucliSENS® easyMag®
- Roche MagNA Pure System
- TANBead Maelstrom 4800 and 9600
- Thermo Fisher KingFisher Flex
- Bruker GenoXtract 12
- (and other compatible systems)
Real-Time PCR Thermocyclers:
- Bio-Rad CFX96 Touch™
- Roche LightCycler® 96, 480, COBAS® z480
- Agilent Aria Mx
- Thermo Fisher ABI 7500 / 7500 Fast
- Azure Cielo 6
- (and other qPCR platforms with FAM/Cy5/HEX/ROX channels)
Related Products
- Virplex® Monkeypox RealTime PCR Kit – RTPCR018-R
- AMPLIRUN® Monkeypox Virus DNA Control – MBC146-R
- AMPLIRUN® Total Monkeypox Virus Control (Swab) – MBTC032-R
The Virplex® MPOX Clades RealTime PCR Kit offers a robust, flexible, and efficient molecular solution for detecting and differentiating MPOX virus clades. With its multiplex format, lyophilized reagents, and wide platform compatibility, it is an essential tool for research laboratories, outbreak surveillance, and molecular virology studies.