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Thermo Scientific™ IPTG Solution, ready-to-use, 10 x 1.5 mL
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Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₉H₁₈O₅S |
Convenient, pre-diluted inducer for lac operon–controlled gene expression
The Thermo Scientific™ IPTG Solution is a highly stable, ready-to-use inducer of the lac operon, formulated at 0.1 M in liquid form for direct application in protein expression workflows. IPTG (isopropyl-β-D-thiogalactopyranoside) is a synthetic lactose analog that inactivates the lac repressor, enabling transcription of lacZ and other genes under lac promoter control.
In molecular cloning, IPTG is commonly used with X-Gal to facilitate blue/white colony screening, allowing clear differentiation between recombinant and non-recombinant bacterial colonies. In protein expression systems, IPTG is essential for inducing recombinant protein production in E. coli strains carrying IPTG-inducible plasmids.
Key Features
- Pre-diluted & Ready-to-Use – Eliminates the need for preparation, reducing handling time and variability
- Highly Stable – Long-term storage at –20 °C without loss of activity
- Consistent Induction – Reliable activation of lac operon–controlled genes
- Dual Use – Ideal for blue/white screening and protein expression induction
- Convenient Packaging – Supplied in 10 × 1.5 mL aliquots to minimize freeze–thaw cycles
Applications
- Blue/white colony screening for recombinant clone identification
- Induction of recombinant protein expression in IPTG-inducible bacterial systems
- Functional studies of lac operon regulation
- Gene expression analysis under lac promoter control
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | R1171 |
| Chemical Name | Isopropyl-β-D-thiogalactopyranoside |
| Concentration | 0.1 M |
| Form | Liquid |
| Quantity | 10 × 1.5 mL |
| Detection Method | N/A (inducer reagent) |
| Storage Conditions | –20 °C |
| Shipping Conditions | Ambient |
| Regulatory Status | Research Use Only (RUO) |
Usage Notes
- For blue/white colony screening, use a final concentration of 0.1 mM IPTG in LB agar or broth.
- Compatible with both solid and liquid culture induction protocols.
- Store at –20 °C and avoid repeated freeze–thaw cycles.
The Thermo Scientific™ IPTG Solution, ready-to-use delivers convenience, reliability, and reproducibility in lac operon–based systems. Whether for colony screening or high-level protein expression, its pre-aliquoted format ensures optimal performance and minimal preparation time.