Thermo Scientific™ DNA Loading Dye & SDS Solution (6X), 5 x 1 mL

The Thermo Scientific™ 6X DNA Loading Dye & SDS Solution is designed for use in agarose gel analysis of DNA samples that contain high amounts of DNA-binding proteins. The solution includes SDS, which eliminates DNA-protein interactions, preventing the appearance of extra bands due to annealing of DNA molecules with cohesive ends. The EDTA component protects DNA from degradation by metal-dependent nucleases during analysis.

Key Features & Benefits:

1. SDS for DNA-Protein Interaction Elimination:
   • The 1% SDS (sodium dodecyl sulfate) in the solution helps break up DNA-protein interactions, ensuring that DNA moves freely through the gel, and prevents any unwanted gel-shifts.
2. EDTA for DNA Protection:
   • The presence of 100 mM EDTA protects DNA from degradation by metal-dependent nucleases, making it ideal for long-term storage and preventing degradation during sample processing.
3. Suitable for High DNA-Protein Samples:
   • Especially useful for analyzing DNA samples containing high levels of DNA-binding proteins, where SDS helps to eliminate the potential interference from these proteins.
4. Versatile Gel Compatibility:
   • This loading dye is compatible with both acrylamide gels and agarose gels, providing flexibility for various types of gel electrophoresis.
5. Consistent and High-Quality Results:
   • The inclusion of bromophenol blue and xylene cyanol FF (XCFF) ensures clear tracking of the DNA during electrophoresis, making it easier to monitor migration in both agarose and acrylamide gels.

Applications:

• DNA Analysis: Ideal for analyzing DNA samples that contain a high concentration of DNA-binding proteins, such as restriction digests, ligation reactions, or dephosphorylation reactions.
• Kinetic Experiments: Useful in experiments involving DNA-binding protein interactions, where the SDS solution minimizes unwanted interactions that could affect migration.
• Routine Agarose Gel Analysis: Commonly used for routine DNA gel analysis, especially when dealing with high-protein DNA preparations that need to be separated based on size.

Specifications:

Storage:

• Store at room temperature. Ensure the product is tightly sealed to prevent contamination and evaporation. The product should be used as part of an electrophoresis procedure involving DNA samples.

The Thermo Scientific™ 6X DNA Loading Dye & SDS Solution is an essential tool for agarose gel electrophoresis, providing enhanced sample integrity by eliminating DNA-protein interactions and protecting DNA from degradation. It is perfect for applications involving DNA-binding proteins, and is compatible with a wide range of gel types for consistent and reliable results.