Promega GoTaq® G2 Hot Start Master Mix

The Promega GoTaq® G2 Hot Start Master Mix is a high-fidelity, ready-to-use PCR master mix designed for reliable and robust DNA amplification. It contains the GoTaq® G2 Hot Start Polymerase, a proprietary antibody-bound enzyme that remains inactive at room temperature and is activated at ≥70°C, ensuring high specificity and reduced nonspecific amplification.

This hot-start PCR master mix simplifies reaction setup while minimizing primer-dimer formation and nonspecific products, making it ideal for sensitive and high-yield PCR applications in molecular biology, genetics, forensics, and diagnostics.

Key Features & Benefits

✅ Room-Temperature Setup

• Hot-start enzyme activation prevents nonspecific amplification and primer-dimers during setup.
• No need for cold reaction preparation, enabling high-throughput and automation-friendly workflows.

✅ Ready-to-Use Master Mix

• Contains GoTaq® G2 Hot Start Polymerase, optimized buffer, dNTPs, and magnesium.
• Just add primers and template DNA, making PCR setup fast and easy.

✅ High Specificity & Enhanced Yield

• Prevents background amplification, leading to higher sensitivity and reproducibility.
• 5′→3′ exonuclease activity ensures robust performance across various targets.

✅ Two Formats for Different Applications

• GoTaq® G2 Hot Start Green Master Mix: Includes gel loading dye for direct gel electrophoresis analysis.
• GoTaq® G2 Hot Start Colorless Master Mix: Does not contain gel loading dye, making it ideal for fluorescence-based or absorbance-based downstream applications (e.g., qPCR, sequencing, fluorescence detection).

✅ Performance Guaranteed

• Validated across various templates and PCR conditions for high consistency and efficiency.
• Tested against leading competitors, ensuring better or equivalent performance.

Technical Specifications

Use Cases & Applications

1. High-Specificity PCR for DNA Amplification

Primary Use:

• Genomic DNA (gDNA), cDNA, and plasmid amplification.
• Multiplex PCR and complex template amplification.

Practical Example:

A genetics research lab studying disease-associated mutations uses GoTaq® G2 Hot Start Master Mix to amplify exonic regions of the BRCA1 gene. The hot-start feature prevents non-specific bands, ensuring clean and accurate results.

2. High-Throughput & Automated PCR Workflows

Primary Use:

• Enables bulk PCR reaction setup without ice or cold room preparation.
• Ideal for high-throughput applications and automated liquid handling systems.

Practical Example:

A forensic DNA lab processing hundreds of crime scene samples daily uses the GoTaq® G2 Hot Start Master Mix (Colorless format) in an automated PCR workflow, ensuring high specificity and reproducibility for STR profiling.

3. Direct Gel Electrophoresis Analysis

Primary Use:

• The Green Master Mix contains a gel loading dye, allowing direct electrophoresis after PCR.
• Eliminates the need for additional loading buffers, reducing steps in PCR workflows.

Practical Example:

A molecular biology teaching lab running student PCR experiments uses the GoTaq® G2 Hot Start Green Master Mix to amplify DNA from plant leaf extracts. Students can directly load their PCR products onto an agarose gel, simplifying analysis.

4. Fluorescence-Based & qPCR Applications

Primary Use:

• The Colorless Master Mix is optimized for fluorescence detection-based applications.
• Ideal for qPCR, sequencing, and probe-based detection methods.

Practical Example:

A biotech company developing a novel qPCR-based diagnostic test selects the GoTaq® G2 Hot Start Colorless Master Mix to avoid interference in fluorescence detection assays, ensuring high sensitivity and precise quantification.

5. Cloning, Mutagenesis & Molecular Engineering

Primary Use:

• Amplification of cloning and site-directed mutagenesis products.
• High-fidelity performance for TOPO cloning, TA cloning, and restriction enzyme digestion workflows.

Practical Example:

A biotechnology startup developing CRISPR gene-editing tools uses GoTaq® G2 Hot Start Master Mix for precise amplification of CRISPR guide RNA templates, ensuring high cloning efficiency and minimal errors.

Ordering Information

Why Choose GoTaq® G2 Hot Start Master Mix?

🔥 Hot-Start Activation – Prevents nonspecific amplification, improving PCR specificity.

🔬 Room-Temperature Setup – Ideal for automated workflows and high-throughput labs.

⚡ Pre-Mixed Convenience – No separate buffer or dNTPs required, just add primers and DNA.

🧪 Multiple Formats Available – Choose Green Mix for direct gel analysis or Colorless Mix for qPCR and fluorescence assays.

📈 Performance-Tested – Proven to outperform or match leading competitor PCR mixes.

🛡 Reliable & Guaranteed – Promega offers a performance guarantee for consistent, high-quality results.

The Promega GoTaq® G2 Hot Start Master Mix is a versatile, high-performance PCR solution that delivers superior specificity, convenience, and efficiency. Whether you need routine PCR, high-throughput automation, fluorescence-based detection, or direct gel analysis, this master mix provides exceptional performance and reliability.