Percoll™ PLUS density gradient media

Cytiva Percoll™ PLUS is a colloidal silica-based density gradient medium designed for preparation, purification, and isolation of cells, subcellular particles, and large viruses.

Each silica particle is coated with silane, providing a non-toxic, inert surface that maintains biological compatibility and iso-osmotic conditions throughout centrifugation.

Percoll™ PLUS builds upon the trusted performance of the original Percoll™ formulation, offering verified low endotoxin levels, greater stability, and enhanced reproducibility. Its low viscosity and low density allow cell separation under gentle centrifugal forces (200–1000 ×g), preserving cell integrity and functionality.

Key Features

• Enhanced Purity: Verified low endotoxin level ensures minimal biological interference.
• Wide Density Range: Iso-osmotic gradients covering densities up to 1.3 g/mL.
• Gentle Cell Isolation: Enables separation under low centrifugal forces (200–1000 ×g), preserving viability.
• Non-Toxic and Inert: Chemically stable, non-ionic, and does not adhere to biological membranes.
• Long-Term Stability:
  • Unopened bottles: Stable for 5 years.
  • Preformed gradients: Maintain shape for weeks (if unfrozen).
• Broad pH Compatibility: Bufferable across pH 5.5–10.0 without altering performance.
• Re-Sterilizable: Can be autoclaved at 120°C for 30 minutes.
• Convenient Packaging: Supplied in easy-to-open, resealable bottles (250 mL or 1 L).

Composition & Physical Properties

Applications

• Cell Biology: Isolation of lymphocytes, neutrophils, macrophages, and other cell types.
• Subcellular Fractionation: Separation of mitochondria, nuclei, lysosomes, and peroxisomes.
• Virology: Purification of large viruses and subviral particles.
• Analytical and Preparative Centrifugation: Formation of continuous or discontinuous gradients.
• Macromolecular Studies: Fractionation of organelles, proteins, and complexes for biochemical research.

Advantages

• Improved gradient stability and reproducibility compared to conventional Percoll™.
• Non-cytotoxic and biocompatible, suitable for live cell recovery.
• Compatible with a wide range of buffers and biological media.
• Flexible gradient formation: Continuous or stepwise gradients possible via centrifugation or gradient mixers.
• High recovery efficiency for delicate cells and particles.

Cytiva Percoll™ PLUS is a next-generation density gradient medium engineered for high-purity and gentle separation of biological materials.

With low endotoxin content, wide density range (up to 1.3 g/mL), and stable, reproducible gradients, it delivers reliable results in cell biology, virology, and biochemical fractionation.

This non-toxic, silane-coated colloidal silica formulation ensures high sample viability, flexible gradient formation, and long-term storage stability, making it the preferred medium for advanced research and cell separation workflows.