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PEIpro® DNA transfection reagent for virus production
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Specifications:
| Application | Transfection | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Sartorius | ||
| Product Grade | Cell Culture | ||
PEIpro® is a next-generation linear polyethylenimine (PEI)-based transfection reagent developed specifically for high-efficiency DNA delivery into suspension-adapted mammalian cells. It is ideal for transient gene expression (TGE) applications in research and commercial-scale viral vector production, such as lentivirus and AAV, as well as recombinant protein manufacturing.
Designed to meet the demanding requirements of bioprocess development and GMP-compatible production workflows, PEIpro® supports high reproducibility, safety, and scalability from milliliter-scale assays to bioreactor-based production runs.
Key Features
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Optimized for Virus Production
PEIpro® offers superior performance for the production of lentiviral and adeno-associated viral (AAV) vectors, achieving high transfection efficiency and viral titers in mammalian cell cultures such as HEK293, HEK293T, and derivative lines. -
Scalable from R&D to Production
Its linear, fully synthetic formulation allows seamless scalability across various volumes and platforms—from small shake flasks to large stirred-tank bioreactors—without protocol modification. -
High Quality and Lot-to-Lot Consistency
Each batch of PEIpro® is rigorously tested and certified for:- Transfection efficiency in HEK293 cells
- Sterility
- Endotoxin levels
- Absence of animal-derived materials
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Animal-Free, GMP-Compatible Formulation
PEIpro® is produced under an ISO 9001-certified quality system, supporting regulatory compliance and minimizing risks associated with biologics manufacturing. -
Ready-to-Use Format
Pre-sterilized and conveniently packaged, PEIpro® simplifies workflow and saves preparation time in high-throughput or critical production settings.
Typical Applications
- Lentivirus and AAV production for gene therapy research
- Recombinant protein expression in suspension cells
- Transient transfection in HEK293 and CHO cells
- Genome engineering using CRISPR-Cas9 systems
- DNA and siRNA delivery
Ordering Information
| Catalog Number | Volume | Description | Pack Type |
|---|---|---|---|
| 101000017 | 1.5 mL | PEIpro® Transfection Reagent | 1 × 1.5 mL vial |
| 101000033 | 10 mL | PEIpro® Transfection Reagent | 1 × 10 mL bottle |
| 101000026 | 100 mL | PEIpro® Transfection Reagent | 1 × 100 mL bottle |
Larger volumes and bulk packaging options are available upon request.
Storage and Stability
- Store at 2–8°C
- Do not freeze
- Shelf life: 12 months from the date of manufacture
- For research or further manufacturing use only. Not intended for diagnostic or therapeutic applications in humans or animals.
Sartorius PEIpro® represents a powerful, versatile, and scalable solution for researchers and manufacturers seeking reliable transient transfection of mammalian cells. Whether used in R&D labs or in upstream bioprocess development for gene therapy products, PEIpro® consistently delivers high transfection efficiency, superior viral vector yields, and process robustness. Its animal-origin free composition and validated quality make it the reagent of choice for both high-throughput research and clinical-grade vector production.
- Pack Size: 100 mL 10 mL 1.5 mL